Bioinformatics analysis of HBV s gene mutation in blood donor

VernacularTitle:献血者HBVs基因变异的生物信息学分析
Author: Jiacheng FAN ¹ ; Qing LI ¹ ; Xiuli CHEN ¹ ; Shixiang LIU ¹ ; Ye CHEN ¹
Author Information

1. Tai’an Central Blood Bank, Tai’an 271000, China
Publication Type:Journal Article
Keywords: HBV; OBI; s gene; blood donor; bioinformatics
From: Chinese Journal of Blood Transfusion 2024;37(8):933-939
CountryChina
Language:Chinese
Abstract: 【Objective】 To analyze the infectious indicators, HBV s gene mutations and changes in bioinformatics characteristics of HBV DNA+ /HBsAg- blood donor. 【Methods】 A sample of HBV DNA+ /HBsAg- was screened by PCR method, and HBsAg/HBsAb/HBeAg/HBeAb/HBcAb of HBV in the sample were detected by ELISA and chemiluminescence methods. The mutation of the HBVs gene fragment in the sample was sequenced and analyzed, and the bioinformatics analysis was performed using analysis software. 【Results】 The sample showed HBV DNA+, HBsAg-, HBsAb+, HBeAg+, HBeAb- and HBcAb+, with an amino acid unit point mutation (P151L) occurred in the HBV s gene, and the spatial structure changed. 【Conclusion】 The spatial structural changes in the gene sequence of HBVs may be the reason for the appearance of serological HBsAg-/HBsAb+ /HBV DNA+ in the test results.