Analysis of ABO allelic enhancement phenomenon in 20 cases with ABO*AW.37 allele
10.13303/j.cjbt.issn.1004-549x.2024.08.007
- VernacularTitle:20例ABO*AW.37等位基因增强现象的分析
- Author:
Xian HUANG
1
;
Shuangyu LI
;
Tongtong LI
;
Lina WU
;
Jinhui XIE
;
Lei MA
;
Jinghui CHONG
;
Shiping AN
Author Information
1. 天津市血液中心,天津 300110
- Keywords:
ABO subtype;
ABO*AW.37;
allelic enhancement;
gene sequencing
- From:
Chinese Journal of Blood Transfusion
2024;37(8):886-889
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the serological and molecular characteristics of twenty blood samples carrying ABO?AW.37 allele and to analyze ABO allelic enhancement.Methods The ABO phenotype of the twenty samples was de-termined by serological methods and the genotype of 1-7 ABO exons was analyzed by Sanger sequencing.Results Sequen-cing analysis showed that all twenty samples contained a c.940A>G(p.Lys314Glu)mutation of A allele,which was defined as ABO?AW.37.When ABO?AW.37 and B alleles were inherited simultaneously in 9 cases,in forward typing anti-A anti-bodies all agglutinated and the serological phenotype was Aw B.Among the 11 cases with ABO?AW.37 and O alleles inherited simultaneously,there was no agglutination of anti-A in forward typing.For absorption and elution tests,5 cases were weakly positive and the serological phenotype was Ael,while 6 cases were negative for absorption and elution tests and the serologi-cal phenotype was O type.Conclusion Allelic enhancement occured when both ABO?AW.37 allele and B allele were in-herited simultaneously.When ABO? AW.37 was inherited simultaneously with O allele,the serological phenotype was Aelor O type and attention should be paid to blood type identification.
