Analysis of ABO allelic enhancement phenomenon in 20 cases with ABO*AW.37 allele
10.13303/j.cjbt.issn.1004-549x.2024.08.007
- VernacularTitle:20例ABO*AW.37等位基因增强现象的分析
- Author:
Xian HUANG
1
;
Shuangyu LI
1
;
Tongtong LI
1
;
Lina WU
1
;
Jinhui XIE
1
;
Lei MA
1
;
Jinghui CHONG
1
;
Shiping AN
1
Author Information
1. Tianjin Blood Center, Tianjin 300110, China
- Publication Type:Journal Article
- Keywords:
ABO subtype;
ABO*AW.37;
allelic enhancement;
gene sequencing
- From:
Chinese Journal of Blood Transfusion
2024;37(8):886-889
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the serological and molecular characteristics of twenty blood samples carrying ABO*AW.37 allele and to analyze ABO allelic enhancement. 【Methods】 The ABO phenotype of the twenty samples was determined by serological methods and the genotype of 1-7 ABO exons was analyzed by Sanger sequencing. 【Results】 Sequencing analysis showed that all twenty samples contained a c. 940A>G(p.Lys314Glu) mutation of A allele, which was defined as ABO*AW.37. When ABO*AW.37 and B alleles were inherited simultaneously in 9 cases, in forward typing anti-A antibodies all agglutinated and the serological phenotype was AwB. Among the 11 cases with ABO*AW.37 and O alleles inherited simultaneously, there was no agglutination of anti-A in forward typing. For absorption and elution tests, 5 cases were weakly positive and the serological phenotype was Ael, while 6 cases were negative for absorption and elution tests and the serological phenotype was O type. 【Conclusion】 Allelic enhancement occured when both ABO*AW.37 allele and B allele were inherited simultaneously. When ABO* AW.37 was inherited simultaneously with O allele, the serological phenotype was Ael or O type and attention should be paid to blood type identification.
