Serological and RHD genotyping characteristics of RhD-negative populations in primary screening in Beijing
10.13303/j.cjbt.issn.1004-549x.2024.08.005
- VernacularTitle:北京地区初筛RhD阴性人群血清学和RHD基因分型特征研究
- Author:
Ke SONG
1
;
Chunya MA
1
;
Yuanyuan LUO
1
;
Yang YU
1
Author Information
1. Department of Transfusion Medicine, the First Medical Center of Chinese PLA Ceneral Hospital, Beijing 100853, China
- Publication Type:Journal Article
- Keywords:
RhD negative;
RHD gene;
genotyping;
gene frequency;
Beijing
- From:
Chinese Journal of Blood Transfusion
2024;37(8):872-878
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To study the RHD genotyping of primary screening RhD negative population in Beijing, and analyze the serological characteristics of different genotypes and the distribution of Rh phenotypes. 【Methods】 From August 2022 to January 2024, 204 RhD negative samples in our hospital were initially screened by microplate or microcolumn agglutination method. RhD antigen phenotype was identified by saline tube method, and RhD negative confirmation test was performed by microcolumn gel-indirect antiglobulin technology.RHD genotyping was performed by polymerasing chain reaction-sequence specific primmer (PCR-SSP) method and sequencing technology, and the phenotypic frequency and gene frequency were calculated. 【Results】 The distribution of Rh serologic phenotypes in 204 RhD negative samples were 112 cases of ccee (54.90%) > 73 cases of Ccee (35.78%) > 9 cases of CCee (4.41%) > 6 cases of ccEe (2.94%) > 4 cases of CcEe (1.96%). A total of 7 RHD genotypes were detected in this study, including 129 cases of RHD* 01N.01 (63.24%), 44 cases of RHD* 01EL.01 (21.57%), 26 cases of RHD* 01N.03 (12.75%), 2 cases of RHD* 01N.16 (0.98%), 1 case of RHD* 06.03.01 (0.49%), 1 case of weak RHD* 15 (0.49%) and 1 case of RHD* 13.01/RHD* 01N.01 (0.49%). The frequency and phenotype distribution of Rh blood group antigen gene were consistent with Hardy-Weinberg equilibrium (P>0.05). 【Conclusion】 The RHD gene is polymorphic in the primary screening RhD negative population in Beijing, and the most common RhD negative genotype is RHD* 01N.01, and the RHD variant is RHD* 01EL.01.This study may provide data to support the clinical implementation of genotype-matched blood transfusions.
