Prediction of B cell epitopes of CD36 and preparation of MAP
10.13303/j.cjbt.issn.1004-549x.2024.08.002
- VernacularTitle:CD36蛋白B细胞抗原表位的生信预测及其多抗原肽的制备
- Author:
Jing LIU
1
,
2
,
3
;
Xiuzhang XU
;
Haoqiang DING
;
Jing DENG
;
Jiali WANG
;
Yangkai CHEN
;
Wen-Jie XIA
;
Xin YE
Author Information
1. 广州血液中心,广东 广州 510091
2. 广州医科大学输血与血液病研究所
3. 广州市血液安全重点实验室
- Keywords:
platelet;
CD36;
B cell epitope;
MAP
- From:
Chinese Journal of Blood Transfusion
2024;37(8):853-858
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the structure of CD36,search the possible B cell epitopes and prepare multi-antigen peptides(MAP)with B cell epitopes,so as to provide a preliminary experimental basis for the preparation of CD36 antibod-ies using MAP with B cell epitopes.Methods The potential B cell epitopes of CD36 were analyzed by bioinformatics meth-ods,including physical and chemical properties,secondary structure,potential phosphorylation and glycosylation sites.Eight-branch MAP with CD36 B cell epitopes were synthesized by FMOC using polylysine as the core matrix.The purity of MAPs was analyzed by reverse high-performance liquid chromatography chromatography(RP-HPLC),and the molecular weight of MAPs was determined by mass spectrometry.Results CD36 is a stable and hydrophilic alkaline protein,with multiple phosphorylation and glycosylation sites and strong antigenicity,and its secondary structure is mainly characterized by irregular curls.Four potential B cell epitopes were obtained and 4 MAPs containing potential B cell epitopes were pre-pared.RP-HPLC analysis showed that the purity of the MAPs were above 85%,and the molecular weight of 3 MAPs was consistent with the expected theoretical molecular weight.Conclusion CD36 on platelet has strong antigenicity.MAPs con-taining CD36 B cell epitopes can provide the experimental basis for the preparation and related research of CD36 antibodies.
