Prediction of B cell epitopes of CD36 and preparation of MAP
10.13303/j.cjbt.issn.1004-549x.2024.08.002
- VernacularTitle:CD36蛋白B细胞抗原表位的生信预测及其多抗原肽的制备
- Author:
Jing LIU
1
,
2
,
3
;
Xiuzhang XU
1
,
2
,
3
;
Haoqiang DING
1
,
2
,
3
;
Jing DENG
1
,
2
,
3
;
Jiali WANG
1
,
2
,
3
;
Yangkai CHEN
1
,
2
,
3
;
Wenjie XIA
1
,
2
,
3
;
Xin YE
1
,
2
,
3
Author Information
1. Guangzhou Blood Center, Guangzhou 510091, China
2. Institute of Blood Transfusion and Hematology at Guangzhou Medical University
3. Guangzhou Key Laboratory of Blood Safety
- Publication Type:Journal Article
- Keywords:
platelet;
CD36;
B cell epitope;
MAP
- From:
Chinese Journal of Blood Transfusion
2024;37(8):853-858
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To analyze the structure of CD36, search the possible B cell epitopes and prepare multi-antigen peptides(MAP) with B cell epitopes, so as to provide a preliminary experimental basis for the preparation of CD36 antibodies using MAP with B cell epitopes. 【Methods】 The potential B cell epitopes of CD36 were analyzed by bioinformatics methods, including physical and chemical properties, secondary structure, potential phosphorylation and glycosylation sites. Eight-branch MAP with CD36 B cell epitopes were synthesized by FMOC using polylysine as the core matrix. The purity of MAPs was analyzed by reverse high-performance liquid chromatography chromatography(RP-HPLC), and the molecular weight of MAPs was determined by mass spectrometry. 【Results】 CD36 is a stable and hydrophilic alkaline protein, with multiple phosphorylation and glycosylation sites and strong antigenicity, and its secondary structure is mainly characterized by irregular curls. Four potential B cell epitopes were obtained and 4 MAPs containing potential B cell epitopes were prepared. RP-HPLC analysis showed that the purity of the MAPs were above 85%, and the molecular weight of 3 MAPs was consistent with the expected theoretical molecular weight. 【Conclusion】 CD36 on platelet has strong antigenicity. MAPs containing CD36 B cell epitopes can provide the experimental basis for the preparation and related research of CD36 antibodies.
