Construction and efficiency detection of Csf1r-CreERT2 R26REYFP reporter gene mouse based on Cre/Loxp system
- VernacularTitle:基于Cre/Loxp系统的Csf1r-CreERT2 R26REYFP报告基因小鼠的构建及效率检测
- Author:
Xiangling ZHU
1
;
Xuming WU
;
Huihui WANG
;
Yuanyuan ZHOU
;
Anqi WANG
;
Huiru ZHANG
;
Chong LIU
;
Jiajie TU
Author Information
- Keywords: Csf1r-CreERT2; R26REYFP; Cre/LoxP system; CD45; flow cytometry
- From: Acta Universitatis Medicinalis Anhui 2024;59(7):1175-1180
- CountryChina
- Language:Chinese
- Abstract: Objective To construct Csf1r-CreERT2 R26REYFP reporter gene mice and assess the efficacy of Csf1r-CreERT2-mediated enhancement of CSF1R in CD45+cells labeled with yellow fluorescein protein EYFP.Methods Csf1r-CreERT2 mice were crossbred with R26REYFP homozygous mice,and Csf1r-CreERT2R26REYFP mice were identified through PCR and Western Blot analyses.Flow cytometry was employed to evaluate CSF1R tag-efficiency in CD45+cells across different mouse tissues following tamoxifen induction.Results Csf1r-CreERT2 R26REYFP reporter gene mice were acquired.In addition,it was found that Csf1r-CreERT2-mediated EYFP could effectively mark CSF1R in various tissues of mice and CD45+cells in different locations.Compared to the R26REYF P group,the highest labeling efficiency was observed in the brain tissue(P＜0.001),the lowest in the thymus tissue(P＜0.05),and no sig-nificant difference was observed in the spleen tissue.Conclusion Adult Csf1r-CreERT2 mice and R26REYFP mice are effective ways to obtain Csf1r-CreERT2 R26REYFP induced conditional fluorescence mice.Csf1r-CreERT2 can mediate EYFP to effectively trace CSF1R in CD45+cells in different parts of mice.
- Full text:2024091016591298385基于Cre_Loxp系统的...告基因小鼠的构建及效率检测_朱向玲.pdf
