Construction and efficiency detection of Csf1r-CreERT2 R26REYFP reporter gene mouse based on Cre / Loxp system
- Author:
Xiangling Zhu
1
;
Xuming Wu
1
;
Huihui Wang
1
;
Yuanyuan Zhou
1
;
Anqi Wang
1
;
Huiru Zhang
1
;
Chong Liu
1
;
Jiajie Tu
1
Author Information
- Publication Type:Journal Article
- Keywords: Csf1r-CreERT2; R26REYFP; Cre / LoxP system; CD45; flow cytometry
- From: Acta Universitatis Medicinalis Anhui 2024;59(7):1175-1180
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To construct Csf1r-CreERT2 R26REYFP reporter gene mice and assess the efficacy of Csf1r- CreERT2 -mediated enhancement of CSF1R in CD45 + cells labeled with yellow fluorescein protein EYFP．
Methods:Csf1r-CreERT2 mice were crossbred with R26REYFP homozygous mice，and Csf1r-CreERT2 R26REYFP mice were identified through PCR and Western Blot analyses．Flow cytometry was employed to evaluate CSF1R tag-efficiency in CD45 + cells across different mouse tissues following tamoxifen induction.
Results :Csf1r-CreERT2 R26REYFP reporter gene mice were acquired．In addition，it was found that Csf1r-CreERT2 -mediated EYFP could effectively mark CSF1R in various tissues of mice and CD45 + cells in different locations．Compared to the R26REYFP group，the highest labeling efficiency was observed in the brain tissue (P＜0. 001) ，the lowest in the thymus tissue (P＜0. 05) ，and no sig- nificant difference was observed in the spleen tissue．
Conclusion :Adult Csf1r-CreERT2 mice and R26REYFP mice are effective ways to obtain Csf1r-CreERT2 R26REYFP induced conditional fluorescence mice．Csf1r-CreERT2 can mediate EYFP to effectively trace CSF1R in CD45 + cells in different parts of mice. - Full text:2024091016591298385基于Cre_Loxp系统的...告基因小鼠的构建及效率检测_朱向玲.pdf
