Action and mechanism of GATA2 overexpression regulating the transcriptome expression of chicken preadipocytes
10.19405/j.cnki.issn1000-1492.2024.07.008
- VernacularTitle:过表达GATA2对鸡前脂肪细胞转录表达的调控作用及机制
- Author:
Yingjun WANG
1
,
2
;
Tingting ZHANG
;
Xiaopei WANG
;
Xinbei ZHOU
;
Yuechan CHEN
;
Zhiwei ZHANG
Author Information
1. 石河子大学组织胚胎学教研室,石河子 832000
2. 石河子大学医学院新疆地方与民族高发病教育部重点实验室,石河子 832000
- Keywords:
chicken;
preadipocytes;
GATA2;
RNA-seq;
ChIP-seq;
enrichment analysis;
GESA analysis
- From:
Acta Universitatis Medicinalis Anhui
2024;59(7):1157-1165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the impact of GATA2 overexpression on transcriptional expression in chicken preadipocytes.Methods Chicken preadipocytes ICP1 were cultivated,pCMV-myc-GATA2 or pCMV-myc plasmid was transfected,Western blot was used to verify the overexpression of GATA2 in cells,RNA-seq was used to study the changes in cell transcriptome expression caused by overexpression of GATA2,ChIP-seq was used to study the binding of GATA2 in the genome,Real time PCR was used to verify some differentially expressed genes,ChIP-PCR was used to verify the collection of GATA2 to TAF3 genes.Results Transfection of pCMV-myc-GATA2 allowed overexpression of GATA2 protein in ICP1 cells;overexpression of GATA2 resulted in up-regulation of 942 genes and down-regulation of 840 genes in ICP1 cells(P＜0.05),and enrichment analysis showed that differentially ex-pressed genes were associated with ribosome and mitochondrial structure and function(P＜0.01);GESA analysis showed that the expression of GATA2-overexpressing ICP1 cells showed up-regulation of RNA polymerase Ⅱ tran-scription initiation complex,ribosome biogenesis,propionate metabolism,and oxidative phosphorylation-related gene sets,and down-regulation of histone lysine N-methyltransferase,nuclear transcription repression complex formation,adipokines,and calcium ions signaling pathway-related gene sets.ChIP-seq in ICP1 cells identified 2833 GATA2-binding genomic peaks involving 2018 genes.motif analysis revealed GATA2-binding(T/A)GATA motifs;enrich-ment analysis showed that these genes are involved in embryonic development,signaling,cellular metabolism,and cell-cell interactions.Differently expressed genes and GATA2-binding genes were taken to intersect to obtain 105 genes,and enrichment analysis showed that these genes were associated with transcription,post-transcriptional regu-lation,cell-cell interactions,signaling,and cell cycle(P＜0.001).Conclusion GATA2 can at least bind to the genome of chicken preadipocytes to regulate their transcriptome expression patterns.
- Full text:2024091016441972518过表达GATA2对鸡前脂肪...胞转录表达的调控作用及机制_王英军.pdf
