The effect and mechanism of Prunella vulgaris-Scutellaria barbata herb pairs in the treatment of breast cancer by integrating network pharmacology and in vitro experiments
10.19405/j.cnki.issn1000-1492.2024.07.005
- Author:
Su Liu
1
,
2
;
Hongxiao Chen
3
;
Le Jin
3
;
Huihui Zhang
4
;
Lei Zhang
3
;
Zhaolin Chen
1
,
2
Author Information
1. School of Pharmacy，Anhui Medical University，Hefei 230032
2. Dept of Pharmacy，The First Affiliated Hospital of USTC (Anhui Provincial Hospital) ，Hefei 230001
3. Dept of Pharmacy，The First Affiliated Hospital of USTC (Anhui Provincial Hospital) ，Hefei 230001
4. Institute of Clinical Pharmacology，Anhui Medical University， Key Laboratory of Anti-Inflammatory and Immune Medicine，Hefei 230032
- Publication Type:Journal Article
- Keywords:
network pharmacology;
prunella vulgaris;
scutellaria barbata;
molecular docking;
cell experiments;
mechanism of action
- From:
Acta Universitatis Medicinalis Anhui
2024;59(7):1134-1142
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the mechanism of Prunella vulgaris and Scutellaria barbata herb pair against breast cancer based on network pharmacology and in vitro cell experiments．
Methods :The effective components and targets of Prunella vulgaris and Scutellaria barbata herb pair were screened．GeneCards and OMIM databases were used to find breast cancer targets，and then drug-active ingredient-key target network and protein-protein inter- action (PPI) were constructed. R language was used to perform GO function and KEGG pathway enrichment analy- sis and survival analysis．Then the screened active components and core targets were verified by molecular docking. Cell viability was detected by CCK-8 assay．EdU and flow cytometry were used to detect cell proliferation and apop- tosis．The protein expression levels of p-AKT1，AKT1，β-catenin and c-MYC were detected by Western blot.
Results :Through databases analysis，a total of 36 active components and 105 intersection targets were screened out，the core components were quercetin，luteolin，kaempferol，wogonin and baicalein．Through PPI and survival analysis，the key targets were AKT1，ESR1，CASP3 and MYC．GO analysis contained 4 303 enrichment results，KEGG analysis contained 232 pathways．Molecular docking showed that the core components had strong binding ability with the key targets．Cell experiments showed that the core active ingredient quercetin could inhibit the proliferation of breast cancer cells and promote their apoptosis (P＜0. 05) ，and down-regulate the expression levels of p-AKT1，β-catenin and c-MYC proteins (P＜0. 05) ．
Conclusion :The active components quercetin in Prunella vulgaris and Scutel- laria barbata herb pair may play a role through AKT1 / β-catenin signaling pathway，which provides a scientific refer- ence for the study of its mechanism of action in the treatment of breast cancer.
- Full text:2024091016283643339整合网络药理学和体外实验探...药对治疗乳腺癌的作用及机制_刘苏.pdf
