Development of Microbubble Contrast Agents for High Frequency Ultrasound Microscopy.
10.3348/jkrs.2007.56.5.505
- Author:
Se Jung JUN
1
;
Eun A KIM
;
Sung Hoon PARK
;
Hye Jin LEE
;
Hong Young JUN
;
Seung Jae BYUN
;
Kwon Ha YOON
Author Information
1. Department of Radiology, Wonkwang University School of Medicine, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Ultrasound (US);
Ultrasound (US), contrast media;
Microbubbles;
Animals;
Microsphere
- MeSH:
Acoustics;
Animals;
Contrast Media*;
Dextrans;
Humans;
Microbubbles*;
Microscopy*;
Microspheres;
Serum Albumin;
Serum Albumin, Bovine;
Ultrasonography*
- From:Journal of the Korean Radiological Society
2007;56(5):505-513
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To develop optimal microbubble contrast agents (MBCAs) for performing ultrasound microscopy when examining small animals. MATERIALS AND METHODS: We prepared three types of MBCAs. First, a mixture of three parts of 40% dextran and one part of 5% human serum albumin were sonicated with perfluorocarbon (PFC) (MB1-D40A5P). Second, three parts of 40% dextran and one part of 1% human serum albumin were sonicated with PFC (MB2-D40A1P). Third, all parts of 1% bovine serum albumin were sonicated with PFC (MB3-A1P). We measured the microbubbles' sizes and concentrations with using image analysis software. The acoustic properties of the microbubbles were assessed both in vitro and in vivo. RESULTS: The majority of the MB1-D40A5Ps had a diameter of 2-5 um, the mean diameter of the MB2-D40A1Ps was 2.5 um, and the mean diameter of the MB3-A1Ps was less than 2.0 um. Among the microbubbles, the MB1-D40A5Ps and MB2-D40A1Ps showed increased echogenicity in the abdominal vessels, but the duration of their contrast effect was less than 30 sec. On the contrary, the MB3-A1Ps exhibited strong enhancement in the vessels and their duration was greater than 120 sec. CONCLUSION: A microbubble contrast agent consisting of all parts of 1% serum albumin sonicated with PFC is an effective contrast agent for ultrasound microscopy.
