Evaluation of a canine small intestinal submucosal xenograft and polypropylene mesh as bioscaffolds in an abdominal full-thickness resection model of growing rats.
10.4142/jvs.2013.14.2.175
- Author:
A Jin LEE
1
;
Sung Ho LEE
;
Wook Hun CHUNG
;
Dae Hyun KIM
;
Dai Jung CHUNG
;
Sun Hee DO
;
Hwi Yool KIM
Author Information
1. Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea. hykim@konkuk.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
biological scaffold;
canine small intestinal submucosa (canine SIS);
inflammatory response;
polypropylene mesh;
tensile load test
- MeSH:
Abdominal Wall/*surgery;
Animals;
Biocompatible Materials/*therapeutic use;
Dogs;
Female;
Intestinal Mucosa/cytology/transplantation;
Intestine, Small/cytology/*transplantation;
Polypropylenes/*therapeutic use;
Rats;
Rats, Sprague-Dawley;
Tensile Strength;
Tissue Adhesions;
*Tissue Scaffolds;
Transplantation, Heterologous/*methods;
*Wound Healing
- From:Journal of Veterinary Science
2013;14(2):175-184
- CountryRepublic of Korea
- Language:English
-
Abstract:
We evaluated the biological scaffold properties of canine small intestinal submucosa (SIS) compared to a those of polypropylene mesh in growing rats with full-thickness abdominal defects. SIS is used to repair musculoskeletal tissue while promoting cell migration and supporting tissue regeneration. Polypropylene mesh is a non-resorbable synthetic material that can endure mechanical tension. Canine SIS was obtained from donor German shepherds, and its porous collagen fiber structure was identified using scanning electron microscopy (SEM). A 2.50-cm2 section of canine SIS (SIS group) or mesh (mesh group) was implanted in Sprague-Dawley rats. At 1, 2, 4, 12, and 24 weeks after surgery, the implants were histopathologically examined and tensile load was tested. One month after surgery, CD68+ macrophage numbers in the SIS group were increased, but the number of CD8+ T cells in this group declined more rapidly than that in rats treated with the mesh. In the SIS group, few adhesions and well-developed autologous abdominal muscle infiltration into the SIS collagen fibers were observed. No significant differences in the tensile load test results were found between the SIS and mesh groups at 24 weeks. Canine SIS may therefore be a suitable replacement for artificial biological scaffolds in small animals.
