Network Correlation Analysis Between Components of Shuanghuanglian Injection and Allergy-like Targets
10.13422/j.cnki.syfjx.20241063
- VernacularTitle:双黄连注射剂成分与类过敏靶点的网络关联性分析
- Author:
Weilong ZHANG
1
;
Hong HE
1
;
Ru QIAO
1
;
Peng HE
1
;
Wenjiao LI
1
;
Liangqi ZHANG
1
;
Xiaoxuan LIU
1
;
Siqi HUANG
1
;
Xue PAN
1
;
Fuyuan HE
1
Author Information
1. School of Pharmacy，Hunan Provincial Key Laboratory of Druggability and Preparation Modification of Traditional Chinese Medicine，Supramolecular Mechanism and Mathematic-physics Chracterization for Chinese Materia Medica，Hunan University of Chinese Medicine，Changsha 410208，China
- Publication Type:Journal Article
- Keywords:
Shuanghuanglian;
traditional Chinese medicine（TCM） injection;
anaphylactoid reaction;
network relevance;
quantitative spectroscopy of TCM;
topological network analysis;
high performance liquid chromatography（HPLC）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(19):190-197
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveBased on the network pharmacology system and quantitative spectroscopy of traditional Chinese medicine（TCM） compounds， a topological network analysis method with equilibrium constant as the core was established to further explore the interaction between allergenic components and their network targets in Shuanghuanglian injection（SHLI）， in order to provide new ideas and experimental basis for identifying and screening potential allergens of SHLI. MethodAfter one week of adaptive feeding， 72 SPF-grade SD male rats were randomly divided into blank group， SHLI standard group， Lonicerae Japonicae Flos（LJF） group， Scutellariae Radix（SR） group， Forsythiae Fructus（FF） group， and 7 groups of SHLI matching groups（groups 1-7）， with 6 rats in each group. Rats in each group were administered the drug intravenously and blood samples were taken after steady state， high performance liquid chromatography（HPLC） characterization profiles of the testing drugs and plasma components in each group were established， and the peak area changes of the drugs and plasma components in each group were calculated after the component groups were classified. Enzyme-linked immunosorbent assay（ELISA） was used to determine the changes of immunoglobulin E（IgE）， histamine（HIS）， tryptase（TPS）， total complement（CH50） and terminal complement complex（C5b-9） in animal blood samples. MATLAB R2020b v9.9.0 software was used to calculate the network balance constants of the component groups with the targets， and the eigenvalues of the matrices composed of network equilibrium constants were calculated and ranked according to their values. ResultELISA results showed that， compared with the blank group， groups 1-3 could significantly increase the IgE level， groups 1-2， groups 4-6 and SHLI standard group could significantly increase the HIS level， group 4 could significantly increase the CH50 level， groups 1， 3-4， LJF group and FF group could significantly increase the TPS level， SR group could significantly increase the C5b-9 level， and the differences were all statistically significant（P<0.05）. According to the retention time of chromatographic peaks， it was classified into 6 component groups from C1 to C6 by HPLC. The order of the network balance constants of each component group was C6>C4>C1>C5>C3>C2， indicating that C6 had the greatest effect on the allergic reaction， and was most likely to be the allergen. The sequence of eigenvalues was C2>C5b-9>C3>C1>CH50>C6>C5>IgE>TPS>C4>HIS， indicating that component group C2 had the greatest contribution to the whole network. ConclusionBased on the correlation analysis of SHLI component group and allergy-like target network， this study clarified that component group C6 may be a potential allergen in SHLI， and the component group C2 may be a key node in the mechanism of drug action， which can provide new strategies and methods for the screening of allergens in TCM injections.
