Neuroprotective Effect of Ginsenoside Rb1 on Lipopolysaccharide-induced Neuroinflammation in Mice Based on PI3K/Akt Signaling Pathway
10.13422/j.cnki.syfjx.20240636
- VernacularTitle:人参皂苷Rb1通过PI3K/Akt信号通路对LPS炎症小鼠的神经保护作用
- Author:
Jiayu XIE
1
;
Yushu LIU
1
;
Feiya ZHU
1
;
Minke TANG
1
Author Information
1. School of Chinese Materia Medica， Beijing University of Chinese Medicine， Beijing 102488，China
- Publication Type:Journal Article
- Keywords:
ginsenoside Rb1;
neuroinflammation;
neuroprotection;
glial cells
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(19):81-89
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the neuroprotective effects of ginsenoside Rb1 on lipopolysaccharide （LPS）-induced neuroinflammation in mice and to preliminarily investigate its mechanism of action. MethodSeventy ICR mice were randomly divided into blank group， model group， dexamethasone sodium phosphate injection group， and low-dose， high-dose ginsenoside Rb1 groups， with 14 mice in each group. A mouse brain neuroinflammation model was prepared using the LPS dose escalation method， starting with a dose of 1 mg·kg-1 and administered via intraperitoneal injection every 48 h （every other morning）. Each subsequent dose increased by 2 mg·kg-1， for a total of 7 injections， culminating in a final dose of 13 mg·kg-1. The dexamethasone sodium phosphate injection group received an intraperitoneal injection at 5 mg·kg-1·d-1. The low-dose and high-dose ginsenoside Rb1 groups were given intraperitoneal injections at 10 mg·kg-1·d-1 and 20 mg·kg-1·d-1， respectively， while the blank and model groups received the same volume of normal saline for 14 days. The behavioral activity of LPS mice was observed， anxiety-like behavior was assessed using the Y-maze and elevated plus maze， and brain levels of monocyte chemoattractant protein-1 （MCP-1）， tumor necrosis factor-α （TNF-α）， and interleukin-1β （IL-1β） were measured by enzyme-linked immunosorbent assay （ELISA）. Neuronal damage of microglia， and the activation status of microglia and astrocytes in the brain were assessed using immunofluorescence staining. The protein expression of phosphatidylinositol-3-kinase （PI3K）， protein kinase B （Akt）， and nuclear factor-κB （NF-κB） in mouse brain were detected by Western blot. ResultCompared with the blank group， the model group showed significantly increased anxiety-like behavior in the Y-maze and elevated plus maze （P<0.05， P<0.01）， significantly elevated levels of MCP-1， TNF-α， and IL-1β in the brain （P<0.01）， a significant decrease in the number of neuronal positive cells in the somatosensory cortex and hippocampus CA1 region （P<0.01）， significant activation of microglia and astrocytes （P<0.01）， and a significant increase in the expression of phosphorylated PI3K， Akt， and NF-κB proteins （P<0.01）. Compared with the model group， the ginsenoside Rb1 low-dose and high-dose groups showed significantly reduced anxiety-like behavior in the Y-maze and elevated plus maze （P<0.05， P<0.01）， significantly decreased levels of MCP-1， TNF-α， and IL-1β in the brain （P<0.01）， a significant increase in the number of neuronal positive cells in the somatosensory cortex and hippocampus CA1 region （P<0.01）， significant inhibition of microglia and astrocyte activation （P<0.05， P<0.01）， and a significant decrease in the expression of phosphorylated PI3K， Akt， and NF-κB proteins （P<0.05， P<0.01）. ConclusionGinsenoside Rb1 has neuroprotective effects on LPS-induced inflammation in mice， which may involve the regulation of the PI3K/Akt signaling pathway.
