Preparation and quality control of antiserum against varicella-zoster virus
10.13200/j.cnki.cjb.004285
- VernacularTitle:水痘-带状疱疹病毒抗血清的制备及检定
- Author:
LIU Hongtao
- Publication Type:Journal Article
- Keywords:
Varicella-zoster virus(VZV);
Neutralizing antibody;
Rabbit antiserum
- From:
Chinese Journal of Biologicals
2024;37(8):983-988
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo prepare varicella-zoster virus(VZV)harvest and immunize Japanese rabbits with purified bulk of virus to prepare antiserum against VZV,and to perform various controltests.MethodsThe VZV Oka strain(vOka)was subcultured in human diploid cell line 2BS. The harvested virus was purified by ultracentrifugation to prepare immunogen,which was mixed with Freund′s adjuvant and then used to immunize 5 female Japanese white rabbits by multi-point subcutaneous injection to prepare VZV antiserum. The specificity of serum antibody was detected by Western blot. The titers of fluorescent antibody to membrane antigen(FAMA)and serum neutralizing antibody were determined,and the correlation between the results of the two detection methods was analyzed. Interference of heterogenous viruses was performed on measles,mumps and rubella viruses by using antiserum with high antibody titer.ResultsThe prepared VZV antiserum had specific binding with vOka and Oka-7S virus harvests,gE and ORF7 proteins and other VZV antigens. After the third immunization,the serum antibody titer detected by FAMA was 1 ∶ 16 384,and the neutralizing antibody titer was 1 ∶ 256.According to Karl Pearson correlation coefficient analysis,the results of the two detection methods were linearly correlated. Interference of heterogenous viruses showed that the differences between the experimental and control groups were less than 0. 50 lgCCID50/mL,indicating that the antisera against VZV had no interference to the titers of measles,mumps and rubella viruses.ConclusionThe VZV antiserum with high specificity and affinity was successfully prepared,which lays a foundation for the validation of VZV component vaccines.
