转化生长因子-β2在小鼠过敏性气道炎症及重塑中的作用与干预潜能
10.13200/j.cnki.cjb.003905
- VernacularTitle:Roles of transforming growth factor-β2 in allergen-induced airway inflammation and remodeling of mice and its potentials as an intervention target
- Author:
YAN Jiao
- Publication Type:Journal Article
- Keywords:
Transforming growth factor-β2(TGF-β2);
Virus-like particles(VLPs);
Ovalbumin(OVA);
Airway inflammation;
Fibrosis;
Asthma
- From:
Chinese Journal of Biologicals
2024;37(8):938-944+951
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the roles of transforming growth factor-β2(TGF-β2)in allergen-induced asthmatic airway inflammation and remodeling and its potentials as an intervention target.MethodsSix female BALB/c mice were immu-nized with hepatitis B virus core antigen(HBcAg)/TGF-β2 virus-like particles(VLPs)presenting TGF-β2epitope. The TGF-β2 specific IgG response in serum of mice was detected by ELISA,and the neutralizing activity of antibody against TGF-β2 was detected by cell test. Acute airway allergic inflammation was induced by a short-term stimulation procedure of ovalbumin(OVA),and the activity of TGF-β in bronchoalveolar lavage fluid(BALF)of mice was detected by cell test. ELISA was used to detect the level of OVA-specific IgE in serum of mice. The total number and type of inflammatory cells in BALF were counted under optical microscope,and the levels of cytokines were detected by ELISA.After sustained stimulation of OVA to induce airway fibrosis,the collagen deposition was detected by Masson′s trichrome staining in lung tissue sections,and the soluble collagen content was quantitatively analyzed by Sircol collagen assay kit.ResultsVLPs immunization induced TGF-β2 specific IgG response,and the antiserum effectively neutralized TGF-β2activity. In the mouse model of acute airway allergic inflammation,VLPs immunization down-regulated the level of active TGF-β in airway,significantly elevated the production of OVA-specific IgE in serum,suppressed the infiltration of airway inflammatory cells mainly containing eosinophils,and significantly promoted the accumulation of Th2 inflammatory cytokines in airway. In chronic pulmonary fibrosis model,VLPs immunization significantly down-regulated collagen deposition and soluble collagen level in lung tissue.ConclusionHBcAg/TGF-β2 VLPs immunization was an effective means to regulate the effect of TGF-β2. TGF-β2 basically showed suppressive effects on airway inflammatory response induced by allergens,while promoted the fibrosis.
