Susceptibility comparison of enterovirus isolation and culture using RD,KMB-17 and Vero cells
10.13200/j.cnki.cjb.004272
- VernacularTitle:RD、KMB-17及Vero细胞对肠道病毒分离培养敏感性的比较
- Author:
LIU Yuhan
- Publication Type:Journal Article
- Keywords:
Enterovirus(EV);
Cell susceptibility;
Vero cells;
RD cells;
KMB-17 cells
- From:
Chinese Journal of Biologicals
2024;37(8):917-920
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo compare the susceptibility of human rhabdomyosarcoma RD cells,human embryonic lung diploid KMB-17 cells and Vero cells to enterovirus(EV)isolation and culture,in order to provide experimental basis for subsequent isolation of EV genotypes.MethodsSixty stool samples of patients with hand-foot-mouth disease(HFMD)diagnosed in a hospital in Kunming in 2019 were collected,and EV strains were isolated and cultured with RD,KMB-17 and Vero cells respectively. The positive samples were detected by RT-PCR,sequenced and blasted by using NCBI BLAST software to identify EV genotypes.ResultsA total of 112 EV strains were isolated,belonging to 11 genotypes respectively. Among them,26strains of Echo-11,6 strains of Echo-6 and Echo-30,2 strains of CV-A4 and CV-A10,1 strain of CV-A6,CV-A16 and Echo-18 were isolated from RD cells;42 strains of Echo-11,3 strains of Echo-6 and 2 strains of Echo-30 were isolated from KMB-17 cells;6 strains of CV-B1,5 strains of CV-B3,4 strains of Echo-11,3 strains of CV-A16 and 1 strain of CV-B2were isolated from Vero cells.ConclusionRD cells were susceptible to most EV genotypes,KMB-17 and Vero cells were the most susceptible to Echo virus and CV-B virus,respectively.
