Preparation of high quality retinal paraffin sections of mouse AMD model and human embryonic eye based on a novel fixative
- VernacularTitle:基于新型固定液制备小鼠AMD模型和人胚胎眼球高质量视网膜石蜡切片
- Author:
Shuantao SUN
1
;
Shuanshuan LIU
;
Shaojun WANG
Author Information
- Keywords: novel fixative; mouse AMD model; human embryonic eye; retina; paraffin section
- From: Acta Universitatis Medicinalis Anhui 2024;59(6):1095-1097
- CountryChina
- Language:Chinese
- Abstract: C57/B6J mice were fixed in traditional fixative(4％formaldehyde solution,the same below)and novel fixative respectively,and the routine procedure of paraffin section preparation was performed,as well as the novel fixative was validated in HE-stained paraffin sections of the eyes of mice and human embryos in the age-related macular degeneration(AMD)model.Compared with the traditional fixative,the quality of the paraffin sections treated with the novel fixative was better than that of the traditional fixative,and successfully simulated the changes of the retinal layers of AMD mice at different time periods.The novel fixative is feasible for the preparation of high-quality retinal paraffin sections,which lays the foundation for subsequent studies on retinal paraffin sections.
- Full text:2024082016305061867基于新型固定液制备小鼠AM...胎眼球高质量视网膜石蜡切片_孙拴桃.pdf
