The total flavonoids of rhododendron alleviate rat brain ischemia⁃reperfusion inj ury by inhibiting the TNF⁃α /caspase⁃8/caspase⁃3 signaling pathway
10.19405/j.cnki.issn1000-1492.2024.06.021
- Author:
Xiaohai Yu
1
;
Yu Jin
1
;
Minqiong Sun
1
;
Qianying Guo
2
;
Hui Cong
3
Author Information
1. School of Basic Medicine , Anhui Medical University , Hefei 230032
2. Dept of Pathology , The First Afiliated Hospital of Anhui Medical University , Hefei 230022
3. Dept of Neurology , East Campus , The First Afiliated Hospital of Anhui Medical University , Hefei 231600
- Publication Type:Journal Article
- Keywords:
total flavones of rhododendron;
cerebral ischemia⁃reperfusion;
cerebral ischemic hypoxic injury;
ap⁃ optosis
- From:
Acta Universitatis Medicinalis Anhui
2024;59(6):1047-1052
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the mechanism by which total flavones of rhododendron ( TFR) protect against cerebral ischemia⁃reperfusion ( I/R) injury by inhibiting the TNF⁃α/caspase⁃8/caspase⁃3 signaling pathway .
Methods :The middle cerebral artery occlusion (MCAO) method was used to establish the rat I/R model . Rats were randomly divided into Sham surgery , MCAO , and post⁃I/R intervention with TFR 200 mg/kg (TFR 200 mg/ kg) groups . After establishing the MCAO rat model , rats in the TFR 200 mg/kg group were administered TFR (200 mg/kg) solution for 14 consecutive days following I/R injury surgery . Hematoxylin⁃Eosin (HE) staining was used to observe neurological function scoring , cerebral blood flow assessment , histological examination of brain tissue , assay kits were used to detect lactate dehydrogenase (LDH) and neuron⁃specific enolase (NSE) activities in rat serum . ELISA assay kits was used to measure interleukin⁃1 (IL⁃1) and interleukin⁃6 (IL⁃6) levels , and Western blot and immunohistochemistry were conducted to detect the expression levels of cleaved caspase⁃3 , caspase⁃8 , and TNF⁃α proteins in rat brain tissue 14 days post⁃surgery .
Results :After cerebral ischemia⁃reperfusion treatment , MCAO resulted in abnormal neurological function in rats , significantly increased neurological function scoring index , obvious changes in cerebral tissue histomorphology and cerebral blood flow , significant upregulation of cleaved caspase⁃3 , caspase⁃8 , and TNF⁃α protein expression levels in brain tissue , and significant elevation of LDH , NSE , IL⁃1 , and IL⁃6 levels in serum . Rats in the TFR 200 mg/kg group showed significantly reduced neurological function scoring , significant improvement in cerebral tissue pathological damage , decreased expression levels of cleaved caspase⁃3 , caspase⁃8 , and TNF⁃α proteins in brain tissue , as well as decreased levels of LDH ,NSE , IL⁃1 , and IL⁃6 in serum .
Conclusion :TFR may alleviate cerebral ischemic hypoxic injury by inhibiting the TNF⁃α/caspase⁃8/caspase⁃3 signaling pathway .
- Full text:2024082011411466425杜鹃花总黄酮通过抑制TNF...大鼠脑缺血再灌注损伤的作用_余孝海.pdf
