Killing effect of composite hydrogel containing Rhodium nanoparticles on pancreatic cancer BxPC⁃3 cells
10.19405/j.cnki.issn1000-1492.2024.06.020
- Author:
Ningwei Wang
1
;
Cong Zhang
1
;
Gang Liu
2
;
Yue Yu
1
Author Information
1. Division of Gastroenterology , The First Afiliated Hospital of University of Science and Technology of China , Hefei 230001
2. Dept of Spleen and Stomach , Susong County Traditional Chinese Medicine Hospital , Anqing 246000
- Publication Type:Journal Article
- Keywords:
Rhodium nanometer;
hydrogel;
reactive oxygen species;
pancreatic cancer
- From:
Acta Universitatis Medicinalis Anhui
2024;59(6):1040-1046
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the killing effect of Rhodium nanoparticles loaded composite hydrogel NPN + Rh⁃PEG NPs (NRP) on pancreatic cancer BxPC⁃3 cells .
Methods :Block copolymers were synthesized using atomtransfer radical polymerization (ATRP) , followed by the synthesis of PEG⁃modified Rhodium nanoparticles through an aqueous method . A premixed solution was prepared by ultrasonication and then heated to synthesize the composite hydrogel NRP loaded with nanoparticles . It was then characterized and its catalytic properties were verified . The morphology of Rhodium nanoparticles and the composite hydrogel NRP was characterized by transmission electron microscopy and scanning electron microscopy . The thermal imaging instrument was used to detect the photothermal properties of the composite hydrogel NRP , and then the growth inhibitory effect on BxPC⁃3 cells was observed using the MTT and live⁃dead staining methods . Finally , its biological safety was verified using MTT and blood compatibility testing .
Results:Rh⁃PEG with a particle size of about 10 nm was successfully prepared . The composite hydrogel showed porous structure under cryo scanning electron microscope , and Rhodium was evenly distributed in the composite hydrogel . Under the irradiation of 808 nm near⁃infrared light (NIR) with a laser power of 1 W/cm2 , the ability of 80 μg/ml NRP to generate reactive oxygen species (ROS) was 19 . 6 times that of pure hydrogel (NPN) (P < 0. 05) . Under light conditions , the catalytic decomposition rate of hydrogen peroxide was as high as 96. 8% . Under the irradiation of 808 nm NIR with a laser power of 1 W/cm2 , the temperature of 80 μg/ml NRP could rise to 58. 9 ℃ within 5 minutes . MTT results showed that the survival rate of BxPC⁃3 cells was the lowest , only 14. 8% , after 40 μg/ml NRP was irradiated with 1 W/cm2 of 808 nm NIR . The results of live dead cell staining proved that the cell killing effect of 40 μg/ml NRP under light irradiation was stronger than that without 808 nm NIR irradiation .
Conclusion :The composite hydrogel NRP uniformly loaded with Rhodium nanoparticles can effectively enhance the killing effect on pancreatic cancer BxPC⁃3 cells .
- Full text:2024082011301515629包载铑纳米颗粒的复合水凝胶...BxPC-3细胞的杀伤效应_王宁薇.pdf
