Effect of hypothyroidism in pregnancy on cognitive function in rat offspring
10.19405/j.cnki.issn1000-1492.2024.06.019
- Author:
Yawei Zhang
1
,
2
;
Yuanyuan Cheng
1
;
Min Xu
1
;
Dake Huang
3
;
Datong Deng
1
Author Information
1. Dept of Endocrinology, The First Afiliated Hospital of Anhui Medical University, Hefei 230022
2. School of Health and Retirement , Anhui City Management Vocational College , Hefei 230011
3. Anhui Medical University Basic Medical College , Hefei 230032
- Publication Type:Journal Article
- Keywords:
hypothyroidism;
pregnancy;
offspring;
hippocampus;
cognitive function
- From:
Acta Universitatis Medicinalis Anhui
2024;59(6):1035-1039
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effects of hypothyroidism and drug intervention on the cognitive function of
rat offspring through the establishment of a rat model of experimental hypothyroidism in pregnancy.
Methods :A total of 20 SD female rats were randomly divided into control group ( CON group) and hypothyroid group ( PTU group) . The hypothyroid model was established by propylthiouracil ( PTU) , the thyroid hormone levels of female mice were detected by electrochemiluminescence immunoassay , and the differences between the two groups were compared. After successful modeling , the male mice were mated in cages , and the hypothyroid group was randomly divided into no intervention group ( group I) , first trimester intervention group ( group II) and second and third trimester intervention group ( group III) . The Morris water maze ( MWM) experiment was used to test the learning and memory ability of the rats. The morphological structure of hippocampal neurons , the expression of nucleoprotein (NeuN) and synapse⁃associated protein ( SYN) in mature neurons were observed by hematoxylin⁃eosin staining (HE) , Nissl staining and immunohistochemistry.
Results :① Compared with the CON group , the female mice in the PTU group had a significant increase in TSH and a significant decrease in FT4 (P < 0. 05) . ② In the positioning navigation test , the evasion latency of the pups in each group was gradually shortened. On the 5th day , the incubation period of group I was significantly longer than that of groups CON , II , III (P < 0. 05) . There was little change between groups II and III and CON groups (P > 0. 05) . ③ The residence time of group II was significantly different from that in group I during the space exploration stage (P < 0. 05) . There was a significant difference between the number of pups crossing the platform in group I and groups CON , II and III (P < 0. 05) . ④ There was no significant difference between HE staining and Nissl staining in hippocampal tissues of rats in each group. However, compared with the CON group , the average absorbance of NeuN and SYN proteins in the hippocampus of mice in groups I , II and III was significantly reduced (P < 0. 05) .
Conclusion :Hypothyroidism will have adverse effects on the cognitive function and hippocampal neuron development of pregnant rats , and the effects of intervention on the cognitive function of offspring at different stages of pregnancy are different , the earlier the intervention , the smaller the damage to cognitive function.
- Full text:2024082011210733389妊娠期甲状腺功能减退对大鼠子代认知功能的影响_张雅玮.pdf
