MiR-19a affects hemangioma stem cells proliferation and migration by targeting insulin-like growth factor 2 receptor

VernacularTitle:miR-19a靶向胰岛素样生长因子2受体抑制血管瘤干细胞的增殖和迁移
Author: Fan WANG ¹ ; Yao WU ; Linsen FANG ; Dongsheng CAO
Author Information

1. 安徽医科大学第一附属医院北区(安徽省公共临床中心)创面修复与整形美容外科,合肥 230001
Keywords: hemangioma stem cells; microRNA; IGF-2R; proliferation; migration
From: Acta Universitatis Medicinalis Anhui 2024;59(6):1029-1034
CountryChina
Language:Chinese
Abstract: Objective To investigate whether miR-19a interacts with insulin-like growth factor 2 receptors(IGF-2R)in infantile hemangiomas(IHs)and affects the proliferation,migration,and adipogenesis of hemangioma stem cells(HemSCs).Methods HemSCs were isolated,screened and cultured from IH specimens.IGF-2R expression in HemSCs was identified using immunohistochemistry.HemSCs transfected with miR-19a mimics and inhibitors were subjected to CCK-8,wound healing,Transwell,qRT-PCR,and Western blot analyses.Results Compared with the control,the proliferation and migration rate of HemSCs treated with miR-19a inhibitors were significantly increased,and overexpression of miR-19a significantly inhibited IGF-2 induced cell migration and proliferation(P＜0.05).Conclusion MiR-19a may inhibit HemSCs proliferation,migration,and adipogenesis by targeting IGF-2R.