MiR⁃19a affects hemangioma stem cells proliferation and migration by targeting insulin⁃like growth factor 2 receptor
10.19405/j.cnki.issn1000-1492.2024.06.018
- Author:
Fan Wang
1
;
Yao Wu
2
;
Linsen Fang
1
;
Dongsheng Cao
2
Author Information
1. Dept of Wound Repair and Plastic Surgery, The First Afiliated Hospital of Anhui Medical University (Anhui Public Health Clinical Center) , Hefei 230001
2. Dept of Plastic Surgery, The Second Afiliated Hospital of Anhui Medical University,Hefei 230001
- Publication Type:Journal Article
- Keywords:
hemangioma stem cells;
microRNA;
IGF⁃2R;
proliferation;
migration
- From:
Acta Universitatis Medicinalis Anhui
2024;59(6):1029-1034
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate whether miR⁃19a interacts with insulin⁃like growth factor 2 receptors ( IGF⁃2R) in infantile hemangiomas (IHs) and affects the proliferation , migration , and adipogenesis of hemangioma stem
cells (HemSCs) .
Methods :HemSCs were isolated , screened and cultured from IH specimens. IGF⁃2R expression in HemSCs was identified using immunohistochemistry. HemSCs transfected with miR⁃19a mimics and inhibitors were subjected to CCK⁃8 , wound healing , Transwell , qRT⁃PCR , and Western blot analyses.
Results :Compared with the control , the proliferation and migration rate of HemSCs treated with miR⁃19a inhibitors were significantly increased , and overexpression of miR⁃19a significantly inhibited IGF⁃2 induced cell migration and proliferation (P < 0. 05) .
Conclusion :MiR⁃19a may inhibit HemSCs proliferation , migration , and adipogenesis by targeting IGF⁃2R.
- Full text:2024082011143564128miR-19a靶向胰岛素样...制血管瘤干细胞的增殖和迁移_汪帆.pdf
