The role and mechanism of palmitoleic acid in the pyroptosis of cardiomyocytes after hypoxia/reoxygenation-induced injury
10.19405/j.cnki.issn1000-1492.2024.06.015
- VernacularTitle:棕榈油酸在缺氧/复氧心肌细胞焦亡中的作用及机制研究
- Author:
Yunquan LI
1
,
2
,
3
;
Zonggang DUAN
;
Hailong BAO
;
Qingteng WANG
;
Hongxin AN
;
Luanda XIAHOU
;
Xu WANG
;
Mengting JIANG
;
Haiyan ZHOU
;
Zhenhua LUO
;
Wei LI
Author Information
1. 贵州医科大学附属医院心内科,贵阳 550004
2. 贵州医科大学临床医学院,贵阳 550004
3. 贵州省人民医院中心实验室,贵阳 550002
- Keywords:
palmitoleic acid;
cardiomyocyte;
hypoxia/reoxygenation-induced injury;
pyroptosis;
inflammation
- From:
Acta Universitatis Medicinalis Anhui
2024;59(6):1006-1012
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of palmitoleic acid(POA)on pyroptosis of cardiomyocytes after hy-poxia/reoxygenation-induced injury in the human myocardium.Methods The experiment comprised a control group(Control,normal culture),a hypoxia/reoxygenation group(HR),a palmitoleic acid-treated group(HR+POA),and an anhydrous ethanol control group(HR+ET).Cardiomyocytes viability was assessed using CCK-8 assay,and the level of pyroptosis of cardiomyocytes was measured through the double staining with Hoechst33342/PI and LDH assay.ELISA was employed to detect the release of inflammatory factors IL-1 β and IL-18 in the cell culture supernatant.qRT-PCR and Western blot were utilized to determine the relative expression levels of mRNA and protein of pyroptosis-related genes,namely NLRP3,ASC,Caspase-1,GSDMD,IL-1 β and IL-18,respective-ly.Results CCK-8 assay showed that the survival of hypoxic/reoxygenated cardiomyocytes increased with the ad-dition of POA at concentrations ranging from 25 to 100 μmol/L,as compared to the HR group;a hypoxia/reoxy-genation model of cardiomyocyte was established.The expression of protein and mRNA increased in NLRP3,ASC,Cleaved caspase-1,GSDMD-N,IL-Iβ and IL-18 vs the control group(P＜0.05),the positive percentage of Ho-echst33342/PI staining in cardiomyocytes increased significantly(P＜0.05),the release of LDH,IL-Iβ,and IL-18 increased(P＜0.05).After intervention with 100 μmol/L POA,the protein and mRNA expression levels of NLRP3,ASC,Cleaved caspase-1,GSDMD-N,IL-Iβ,and IL-18 were significantly reduced in the HR+POA group vs HR+ET group(P＜0.05).The positive percentage of Hoechst33342/PI staining in cardiomyocytes de-creased significantly,and the levels of LDH,IL-Iβ and IL-18 significantly decreased(P＜0.05).Conclusion Palmitoleic acid may alleviate hypoxia/reoxygenation-induced injury of cardiomyocytes by inhibiting pyroptosis and inflammatory response after hypoxia/reoxygenation in human myocardium.
- Full text:202408201037018333棕榈油酸在缺氧_复氧心肌细胞焦亡中的作用及机制研究_李云权.pdf
