The role and mechanism of palmitoleic acid in the pyroptosis of cardiomyocytes after hypoxia/reoxygenation⁃induced inj ury
10.19405/j.cnki.issn1000-1492.2024.06.015
- Author:
Yunquan Li
1
,
2
,
3
;
Zonggang Duan
1
,
2
,
3
;
Hailong Bao
1
,
2
;
Qingteng Wang
1
,
2
;
Hongxin An
1
,
2
;
Luanda Xiahou
1
,
2
;
Xu Wang
1
,
2
;
Mengting Jiang
1
,
2
;
Haiyan Zhou
1
,
2
;
Zhenhua Luo
3
;
Wei Li
1
,
2
Author Information
1. Dept of Cardiovascular Medicine , The Afiliated Hospital of Guizhou Medical University , Guiyang 550004
2. School of Clinical Medicine , Guizhou Medical University , Guiyang 550004
3. Dept of Central Lab , Guizhou Provincial People s Hospital , Guiyang 550002
- Publication Type:Journal Article
- Keywords:
palmitoleic acid;
cardiomyocyte;
hypoxia/reoxygenation⁃induced injury;
pyroptosis;
inflammation
- From:
Acta Universitatis Medicinalis Anhui
2024;59(6):1006-1012
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of palmitoleic acid (POA) on pyroptosis of cardiomyocytes after hypoxia/reoxygenation⁃induced injury in the human myocardium .
Methods :The experiment comprised a control group (Control , normal culture) , a hypoxia/reoxygenation group (HR) , a palmitoleic acid⁃treated group ( HR + POA) , and an anhydrous ethanol control group ( HR + ET) . Cardiomyocytes viability was assessed using CCK⁃8 assay , and the level of pyroptosis of cardiomyocytes was measured through the double staining with Hoechst33342/ PI and LDH assay . ELISA was employed to detect the release of inflammatory factors IL⁃1β and IL⁃18 in the cell culture supernatant . qRT⁃PCR and Western blot were utilized to determine the relative expression levels of mRNA and protein of pyroptosis⁃related genes , namely NLRP3 , ASC , Caspase⁃1 , GSDMD , IL⁃1β and IL⁃18 , respectively .
Results :CCK⁃8 assay showed that the survival of hypoxic/reoxygenated cardiomyocytes increased with the addition of POA at concentrations ranging from 25 to 100 μmol/L , as compared to the HR group ; a hypoxia/reoxy⁃genation model of cardiomyocyte was established . The expression of protein and mRNA increased in NLRP3 , ASC ,Cleaved caspase⁃1 , GSDMD⁃N , IL⁃Iβ and IL⁃ 18 vs the control group(P < 0. 05) , the positive percentage of Hoechst33342/PI staining in cardiomyocytes increased significantly (P < 0. 05) , the release of LDH , IL⁃Iβ , and IL⁃18 increased (P < 0. 05) . After intervention with 100 μmol/L POA , the protein and mRNA expression levels of NLRP3 , ASC , Cleaved caspase⁃1 , GSDMD⁃N , IL⁃Iβ , and IL⁃18 were significantly reduced in the HR + POA group vs HR + ET group( P < 0. 05) . The positive percentage of Hoechst33342/PI staining in cardiomyocytes decreased significantly , and the levels of LDH , IL⁃Iβ and IL⁃18 significantly decreased (P < 0. 05) .
Conclusion:Palmitoleic acid may alleviate hypoxia/reoxygenation⁃induced injury of cardiomyocytes by inhibiting pyroptosis and inflammatory response after hypoxia/reoxygenation in human myocardium .
- Full text:202408201037018333棕榈油酸在缺氧_复氧心肌细胞焦亡中的作用及机制研究_李云权.pdf
