Construction of macrophage⁃specific TDO2 knockout mice
10.19405/j.cnki.issn1000-1492.2024.06.013
- Author:
Weibo Dong
1
;
Yuelan Chen
1
;
Yi Wang
1
;
Meng Cheng
1
;
Wei Wei
1
;
Yan Chang
1
Author Information
1. Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory ofAnti⁃Inflammatory and Immune Medicine , Ministry of Education , Center of Rheumatoid Arthritis of Anhui Medical University, Anhui Collaborative Innovation Center ofAnti⁃inflammatory and Immune Medicine , Hefei 230032
- Publication Type:Journal Article
- Keywords:
TDO2;
specific gene knockout;
macrophage;
genotype identification;
Cre⁃loxP
- From:
Acta Universitatis Medicinalis Anhui
2024;59(6):994-1000
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To provide an animal model for studying the effect of TDO2 on the function of macrophages on the occurrence and development of diseases by constructing macrophage⁃specific tryptophan , 2 , 3 ⁃dioxygenase (TDO2) gene knockout mice.
Methods :TDO2flox/flox Lyz2⁃iCre + mice were constructed based on Cre/LoxP system. The genotypes of mice were identified by PCR amplification and agarose gel electrophoresis. Western blot and immunofluorescence were used to verify the effect of TDO2 knockdown in mouse macrophages. The spontaneous lesions in major tissues and organ were observed by HE stainings.
Results :The results of genotype identification showed that the mice with only one band at 407 bp or 408 bp for the flox amplification product and one band at 543 bp for the Cre amplification product were TDO2flox/flox Lyz2⁃iCre + mice. Western blot results showed that TDO2 expression in bone marrow⁃derived macrophages (BMDMs) of TDO2flox/flox Lyz2⁃iCre + mice decreased compared with TDO2flox/flox mice (P < 0. 01) . Immunofluorescence results showed that TDO2 expression in peritoneal macrophages and BMDMs of TDO2flox/flox Lyz2⁃iCre + mice decreased compared with TDO2flox/flox mice. HE staining showed no significant differences in cell morphology in the liver, brain , kidney and spleen tissues of TDO2flox/flox Lyz2⁃iCre + mice compared to TDO2flox/flox mice.
Conclusion :TDO2flox/flox Lyz2⁃iCre + mice is successfully constructed , providing a more precise experimental animal model for subsequent in⁃depth study of the role and mechanism of TDO2⁃regulated macrophage activation in disease.
- Full text:2024082010095143563巨噬细胞特异性TDO2基因敲除小鼠的构建_董伟波.pdf
