Application of melatonin-supplemented in vitro maturation technology for human oocytes during COH cycle
10.19405/j.cnki.issn1000-1492.2024.06.011
- VernacularTitle:补充褪黑素的人卵母细胞体外培养成熟技术在COH周期中的应用
- Author:
Yu REN
1
,
2
,
3
,
4
;
Xingxing HAN
;
Qiqi ZHANG
;
Lu LIU
;
Xiaofeng XU
;
Zhiguo ZHANG
;
Huijuan ZOU
Author Information
1. 安徽医科大学第一附属医院妇产科,合肥 230032
2. 国家卫生健康委配子及生殖道异常研究重点实验室,合肥 230032
3. 出生人口健康教育部重点实验室,合肥 230032
4. 生命资源保存与人工器官教育部工程研究中心,合肥 230032
- Keywords:
oocyte;
in vitro maturation technology;
in vitro fertilization;
controlled ovarian hyperstimulation;
pregnancy outcome
- From:
Acta Universitatis Medicinalis Anhui
2024;59(6):983-988
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the early embryonic developmental potential and clinical outcomes of oocytes matured in vivo and those matured by modified in vitro maturation(LVM)technology during the same controlled ovarian hyperstimulation(COH)cycle,and to explore the clinical application of melatonin-supplemented IVM technology.Methods 159 patients were recruited into the study.920 mature oocytes were collected during their COH cycles processed for conventional IVF/ICSI protocols,while 1 283 immature oocytes from the same cycles were matured in a melatonin-supplemented IVM medium before ICSI was performed.A retrospective analysis was conducted to compare the impact of conventional assisted reproductive technology and improved IVM technology on the outcomes of assisted reproductive therapy and pregnancy outcomes.Results Compared with mature oocytes collected from COH cycles treated with conventional IVF/ICSI,oocytes promoted by improved melatonin-supple-mented IVM technology had a lower rate of high-quality blastocyst formation.However,after embryo transfer,there was no significant difference in the clinical outcomes of mature oocytes obtained through two methods,including clinical pregnancy rate,full-term birth rate,neonatal length,and neonatal Apgar score.Conclusion The applica-tion of melatonin-supplemented IVM significantly increases the utilization of immature oocytes collected from COH cycles,improving the pregnancy outcomes of patients assisted by assisted reproductive technology.
