Glycyrrhizic acid⁃mediated protective effect of miRNAs on alcoholic liver inj ury in rats
10.19405/j.cnki.issn1000-1492.2023.08.021
- Author:
Meili Cong
1
;
Mayire Nuermaimaiti
1
;
Bei Zhou
1
;
Ruihua Chen
1
;
Zhong Wang
1
;
Huhu Zhu
1
;
Xiaoguo Zhao
1
Author Information
1. Animal Laboratory Center of Xinjiang Medical University , Urumqi 830011
- Publication Type:Journal Article
- Keywords:
alcoholic liver injury;
glycyrrhizic acid;
miRNA;
signaling pathway;
microarray
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1374-1379
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the mechanism of miRNAs in glycyrrhizic acid treatment of alcohol⁃induced
liver injury in rats .
Methods :45 male SD rats were randomly divided into glycyrrhizin group , model group and control group . The rats in glycyrrhizin group were given 56% liquor and glycyrrhizin , the rats in model group were
given 56% liquor , and the rats in control group were given distilled water for 8 weeks . The blood was collected and the serum was separated by centrifugation to detect the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) . RNA were extracted from liver tissues , miRNAs were detected by rat miRNA microarray , and their expression levels were analyzed . The miRNA target genes of differential miRNA were predicted . Gene Ontology (GO) and KEGG Pathway enrichment analysis were used to understand the function of differential miRNA , and the differential miRNA⁃mRNA⁃Pathway regulatory network was constructed using Cytoscape to further screen the regulatory key miRNA and key pathways . qRT⁃PCR was used to verify the expression of selected miRNA .
Results : Compared with the model group , the glycyrrhizin group could significantly improve the liver tissue lesions , and reduce the liver serum AST and ALT levels (P < 0. 05) . Compared the microarray data of glycyrrhizin group and model group , a total of 13 differentially expressed miRNA were screened ( P < 0. 05 , fold change ≥ 1 . 5) , of which 10 were up⁃regulated and 3 were down⁃regulated . The GO classification annotation of differential miRNA target genes showed that differential miRNA were related to cell adhesion , antioxidant activity , metabolic process , biological process regulation , cell killing , immune system and other functions . The KEGG Pathway analyling pathway , Hippo signaling pathway , PI3K⁃Akt signaling pathway , wnt signaling pathway , apoptosis and other signaling pathways might play an important regulatory role in the improvement of alcoholic liver injury by glycyrrhic acid .
Conclusion :This study established the miRNA expression profile of glycyrrhizin in the treatment of alcoholic liver injury in rats , suggested that miR⁃615 , miR⁃107 ⁃3p and miR⁃292⁃5p might play an important role in the treatment of alcoholic liver injury by glycyrrhizin .
- Full text:2024081909582411252甘草酸介导miRNAs对大鼠酒精性肝损伤的保护作用_从美丽.pdf
