The Replication Method and Evaluation of Experimental Animal Models with Insomnia of Liver Depression and Qi Stagnation Syndrome
10.13288/j.11-2166/r.2024.14.013
- VernacularTitle:肝郁气滞型失眠实验动物模型复制方法及评价概述
- Author:
Renzhen ZHANG
1
;
Yujuan YE
1
;
Yuting WEI
1
;
Cui MA
1
;
Xingke YAN
1
Author Information
1. School of Acupuncture，Moxibustion and Tuina，Gansu University of Chinese Medicine，Lanzhou，730000
- Publication Type:Review
- Keywords:
insomnia;
liver depression and qi stagnation;
combination of disease and syndrome;
animal model;
review
- From:
Journal of Traditional Chinese Medicine
2024;65(14):1496-1503
- CountryChina
- Language:Chinese
-
Abstract:
In recent years， the research data on the combination model of insomnia of liver depression and qi stagnation syndrome in traditional Chinese medicine （TCM） were sorted out and summarized in terms of the selection of experimental animals， modelling methods， and model evaluation， with a view to providing references for the standardized preparation of the experimental model of insomnia of liver depression and qi stagnation syndrome and the study of the intervention mechanism. As for the selection of experimental animals， rats and mice are commonly used for the study of sleep patterns in the insomnia model of liver depression and qi stagnation syndrome because their sleep patterns are similar to those of human beings； zebrafish is suitable for the study of sleep patterns in early development because of its high genetic homology； and rhesus macaques， common marmosets， and crab-eating monkeys are used for the study of highly complex sleep disorders because their physiological and behavioural characteristics are closer to those of human beings. In terms of modelling methods， electrical stimulation， chronic restraint， chronic emotional stress and impact psychological stress each have their own characteristics and application scenarios， while chronic tail-clamping stimulation combined with intraperitoneal injection of p-chlorophenylalanine （PCPA） combines physical and chemical stimuli， in order to simulate the complex mechanism of insomnia in human beings， and to more comprehensively simulate the pathology of insomnia with liver depression and qi stagnation syndrome. In terms of evaluation indexes， electroencephalography （EEG） and electromyography （EMG） monitoring and barbiturate synergistic sleep test are the most commonly used and scientific methods for evaluating insomnia animal models with liver depression and qi stagnation syndrome， which can provide intuitive and continuous sleep state monitoring； barbiturate synergistic sleep test can reflect the sensitivity and responsiveness of the model animals to the sleep-regulating medications； and the introduction of the method of using formulas to measure syndromes for the evaluating the model can combine the treatment and diagnostic principles of TCM with modern scientific research.
