Construction of A Macro-evaluation Tool for Dampness Syndrome Animal Model in Traditional Chinese Medicine
10.13288/j.11-2166/r.2024.14.007
- VernacularTitle:中医湿证动物模型宏观评价工具的研制
- Author:
Chuang LI
1
;
Peng XU
1
;
Ruimin TIAN
1
;
Zhaorui CAO
1
;
Mingjia LIU
1
;
Lei ZHANG
1
;
Zhaoyu LU
1
;
Taohua LAN
1
;
Xiaowan WANG
1
;
Wei MAO
1
Author Information
1. State Key Laboratory of Dampness Syndrome of Chinese Medicine，The Second Affiliated Hospital of Guangzhou University of Chinese Medicine，Guangzhou，510120
- Publication Type:Journal Article
- Keywords:
dampness syndrome;
animal model;
model evaluation tool;
expert importance rating
- From:Journal of Traditional Chinese Medicine
2024;65(14):1449-1457
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo construct a macro-evaluation tool for dampness syndrome （DS） animal model， which will provide a basis for experimental research on dampness syndrome in traditional Chinese medicine （TCM）. MethodsConceptual framework of this study was clarified through discussions within the core working group， and dimensions of the evaluation of the animal model of DS were identified according to TCM principles. We searched CNKI， Wanfang， VIP and SinoMed databases from the inception to June 30th， 2023， on experiments involving dampness syndrome animals to create a pool of items about DS animal models. The core items were selected and extracted for factor analysis and cluster analysis. An expert importance rating questionnaire was developed based on the results of the literature review， analyzing the distribution of item scores， importance averages， and coefficient of variation. Through a comprehensive analysis of literature， expert importance scoring， and specific expert opinions， items that did not meet anyone of the criteria of average importance rating ≥2.04， coefficient of variation ≤30%， or literature eva-luation frequency ≥2% were removed， thereafter， the macro-evaluation tool for DS animal model was preliminarily constructed. ResultsSpirit and body state， autonomic activity state， body surface characteristics， diet， urination and defecation， tongue manifestation， and motor behavior assessment were constructed as the seven dimensions in the evaluation of DS animal model. A total of 348 papers about animal experiments were included and analyzed， resulting in a saturated pool of 72 items， which was refined to 38 core items of DS animal models. Factor analysis obtained 16 common factors， which were further clustered into two categories， named dampness transforming from heat syndrome and dampness transforming from cold syndrome. The expert importance scoring showed that the Kendall harmony coefficient was 0.359 （P＜0.05） indicating a high level of agreement， coordination and reliability among the experts. Notably， 50% or more of the experts considered the items thick and greasy tongue coating， unclean perianal area， loose stools， lethargy， unformed stools， and listless expression as very important. The median scores for all items were 2.04（1.73， 2.37）， with a coefficient of variation ranging from 19.73% to 53.38%. After expert evaluation， the macro-evaluation tool for DS animal model in TCM with 33 items and corresponding criteria for assessing the formation of DS models was finally contructed. ConclusionThe Macro-evaluation tool for DS animal model in TCM is highly scientific， credible， and operable， and can be utilized in DS animal experiments after its characteristics are actually evaluated.
