Genotyping-by-sequencing Reveals Genetic Diversity of Artemisia argyi Germplasm Resources
10.13422/j.cnki.syfjx.20240413
- VernacularTitle:基于GBS简化基因组技术的蕲艾遗传多样性分析
- Author:
Changjie CHEN
1
;
Chuang XIAO
1
;
Yuyang MA
1
;
Yuhuan MIAO
1
;
Dahui LIU
1
Author Information
1. College of Pharmacy，Hubei University of Chinese Medicine，Wuhan 430065，China
- Publication Type:Journal Article
- Keywords:
Artemisia argyi;
genotyping-by-sequencing （GBS）;
single nucleotide polymorphism （SNP） marker;
genetic diversity analysis;
cluster analysis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(18):171-177
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the genetic relationship and reveal the genetic variations of 45 germplasm accessions of Artemisia argyi. MethodGenotyping-by-sequencing （GBS） was employed to mine single nucleotide-polymorphisms （SNPs） from the 45 germplasm accessions. Principal component analysis， phylogenetic analysis， population genetic structure analysis， and genetic variation analysis were conducted based on the SNPs. ResultA total of 111.91 Gb of data were obtained， with the Q20， Q30， and average GC content of 96.39%， 90.33%， and 39.37%， respectively. The comparison rate between clean reads and the reference genome was 70.24%-98.97%. A total of 22 399 Indels and 170 539 SNPs were obtained， and the 10th pair of chromosomes had the most variation sites. The results of principal component analysis， cluster analysis， and genetic diversity analysis classified the 45 germplasm accessions into three groups. Group Ⅰ contained three germplasm accessions from Qichun County. The germplasm accessions in group Ⅱ were all wild. Group Ⅲ contained 31 germplasm accessions， with the most complex sources. Moreover， the 45 germplasm accessions can be classified into 3 subtypes， containing the genetic information from three ancestors. The results indicated rich genetic diversity of A. argyi from different sources， especially the germplasm accessions from Qichun County， Hubei province. ConclusionThis study provides theoretical support for breeding new varieties， developing specific SNP markers， and revealing the genetic relationship of A. argyi.
