Effect of Buzhong Yiqitang on Fas/FADD/Caspase-8 Cell Apoptotic Signaling Pathway in Mice with Autoimmune Thyroiditis
10.13422/j.cnki.syfjx.20241044
- VernacularTitle:补中益气汤对自身免疫性甲状腺炎小鼠Fas/FADD/Caspase-8细胞凋亡信号通路的影响
- Author:
Xiaohui LI
1
;
Zhuo ZHAO
1
;
Yiran CHEN
1
;
Huimin CAO
1
;
Si CHEN
1
;
Zhimin WANG
2
;
Tianshu GAO
2
;
Ziyu LIU
3
;
Xiao YANG
3
Author Information
1. Liaoning University of Traditional Chinese Medicine（TCM），Shenyang 110847，China
2. Affiliated Hospital of Liaoning University of TCM， Shenyang110032，China
3. The Second Affiliated Hospital of Liaoning University of TCM，Shenyang 110034，China
- Publication Type:Journal Article
- Keywords:
Buzhong Yiqitang;
autoimmune thyroiditis;
apoptosis;
tumor necrosis factor receptor superfamily member 6 （Fas）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(18):27-34
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of Buzhong Yiqitang on the tumor necrosis factor receptor superfamily member 6 （Fas）/Fas related death domain protein （FADD）/Caspase-8 cell apoptotic signaling pathway in mice model with autoimmune thyroiditis （AIT）. MethodThere were 120 SPF grade NOD.H-2h4 mice aged 8 weeks， 100 of which were fed with high iodine water （0.05% NaI）， and the AIT model was made after 8 weeks. According to random number table method， they were divided into model group， Buzhong Yiqitang low-dose， medium-dose and high-dose groups （4.78， 9.56， 19.12 g·kg-1）， selenium yeast tablet group （3.033×10-5 g·kg-1）， with 20 mice in each group and 20 control group. The apoptosis of thyroid cells was detected by in situ end labeling （TUNEL） after 8 weeks of administration. The real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） was used to detect the mRNA expression of Fas， FADD， Caspase-8， and Caspase-3 in thyroid tissue. Western blot was used to detect the protein expression of Fas， FADD， Caspase-8， Caspase-3， cleaved Caspase-8， and cleaved Caspase-3 in thyroid tissue， and the immunohistochemical method was used to detect the protein expression of Fas and Caspase-3 in thyroid tissue. ResultCompared with control group， there were more positive expressions of apoptotic cells in model group under fluorescence microscope， and the expressions of Fas， FADD， Caspase-8， Caspase-3， cleaved Caspase-8 and cleaved Caspase-3 were significantly increased （P<0.05， P<0.01）. Compared with model group， the positive expression of thyroid apoptotic cells in each administration group was decreased under fluorescence microscope， and the expressions of Fas， FADD， Caspase-8， Caspase-3， cleaved Caspase-8， cleaved Caspase-3 were significantly decreased （P<0.05， P<0.01）. ConclusionBuzhong Yiqitang can effectively improve thyroid cell apoptosis and inflammatory injury in AIT mice. The mechanism may be related to the inhibition of Fas/FADD/Caspase-8 signaling pathway.
