Mechanism of Buzhong Yiqitang in Improving Autoimmune Thyroiditis by Regulating Th17 Cells Through miR-155/Ndfip1/Pten Axis
10.13422/j.cnki.syfjx.20241039
- VernacularTitle:补中益气汤通过miR-155/Ndfip1/Pten轴调控Th17细胞改善自身免疫性甲状腺炎的作用机制
- Author:
Xiaohui LI
1
;
Zhuo ZHAO
1
;
Yiran CHEN
1
;
Huimin CAO
1
;
Si CHEN
1
;
Zhimin WANG
2
;
Tianshu GAO
2
;
Ziyu LIU
3
;
Xiao YANG
3
Author Information
1. Liaoning University of Traditional Chinese Medicine（TCM），Shenyang 110847，China
2. Affiliated Hospital of Liaoning University of TCM， Shenyang110032，China
3. The Second Affiliated Hospital of Liaoning University of TCM，Shenyang 110034，China
- Publication Type:Journal Article
- Keywords:
Buzhong Yiqitang;
autoimmune thyroiditis （AIT）;
helper T cell 17（Th17） cells;
microRNA-155 （miR-155）;
Nedd4 family interacting protein 1 （Ndfip1）;
phosphatase and tensin homolog （Pten）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(18):19-26
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the mechanism of Buzhong Yiqitang in improving autoimmune thyroiditis （AIT） by regulating helper T cell 17（Th17） cells through microRNA-155 （miR-155）/Nedd4 family interaction protein 1 （Ndfip1）/phosphatase and tensin homology （Pten） axis. MethodThe 100 SPF grade 8 week-old NOD.H-2h4 mice were fed with high iodine water （0.05% NaI） for 8 weeks， and AIT model was made. They were divided into model group， Buzhong Yiqitang low-，medium-，and high-dose groups （4.78，9.56，19.12 g·kg-1·d-1） and selenium yeast tablet group （3.033×10-5 g·kg-1） according to random number table method. There were 20 mice in each group and 20 mice in the control group. The control group and the model group were given the same amount of distilled water. After 8 weeks of continuous administration， Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） was used to detect the expression of miR-155-5p， Ndfip1， Pten， protein tyrosine kinase 1 （Jak1）， signaling and transcriptional activator 3 （Stat3） retinoic acid-associated orphan receptor γt （RORγt）， and interleukin-17 （IL-17） mRNA in mouse thyroid tissue. Western blot was used to detect the expression of Ndfip1， Pten， Jak1， Stat3， RORγt， and IL-17 proteins in mouse thyroid tissue， immunohistochemical method was used to detect the expression of Ndfip1 and Pten proteins in mouse thyroid tissue； flow cytometry was used to detect the proportion of Th17 cells in mouse spleen. ResultCompared with the control group， the proportion of Th17 cells was increased （P<0.01）. The expressions of miR-155-5p， Jak1， Stat3， RORγt and IL-17 were increased （P<0.01）， while the expressions of Ndfip1 and Pten were decreased （P<0.01）. Compared with model group， the proportion of Th17 cells was decreased （P<0.05，P<0.01）. The expressions of miR-155-5p， Jak1， Stat3， RORγt and IL-17 were decreased （P<0.05，P<0.01）， while the expressions of Ndfip1 and Pten were increased （P<0.05，P<0.01）. ConclusionThe application of Buzhong Yiqitang can improve the autoimmune disorder of AIT mice， the mechanism of which may be related to the regulation of Ndfip1/Pten axis by miR-155 and then the regulation of Th17 cell differentiation.
