Mechanism of Buzhong Yiqitang in Ameliorating Thyroiditis Damage in AIT Mice by Modulating TLR4/NF-κB/AIM2 Signaling Pathway
10.13422/j.cnki.syfjx.20241038
- VernacularTitle:补中益气汤调控TLR4/NF-κB/AIM2信号通路改善自身免疫性甲状腺炎小鼠甲状腺炎症损伤的作用机制
- Author:
Zhuo ZHAO
1
;
Zhe JIN
2
;
Zhengzheng LI
1
;
Xuanlin GUO
1
;
Jiayun LI
1
;
Tongran GAO
1
;
Pin LI
3
;
Zhimin WANG
3
;
Yuanping YIN
2
;
Ziyu LIU
2
;
Xiao YANG
2
Author Information
1. Liaoning University of Traditional Chinese Medicine（TCM），Shenyang 110847，China
2. The Second Affiliated Hospital of Liaoning University of TCM，Shenyang 110034，China
3. Affiliated Hospital of Liaoning University of TCM， Shenyang 110032，China
- Publication Type:Journal Article
- Keywords:
Buzhong Yiqitang;
Toll-like receptor 4（TLR4）/nuclear transcription factor-kappa B（NF-κB） signaling pathway;
absent in melanoma 2（AIM2） inflammasome;
autoimmune thyroiditis;
inflammation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(18):1-9
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the mechanism of Buzhong Yiqitang in ameliorating inflammatory injury in autoimmune thyroiditis (AIT) mice based on the Toll-like receptor 4（TLR4）/nuclear transcription factor-kappa B（NF-κB）/absent in melanoma 2（AIM2）inflammasome signaling pathway. MethodThe 120 genetically susceptible 8-week-old NOD.H-2h4 mice were selected and randomly divided into control group， model group， low， medium and high dose groups of Buzhong Yiqitang （4.78， 9.56， 19.12 g·kg-1）， and western medicine group （selenium yeast tablets， 3.033×10-5 g·kg-1）. The AIT model mice in each group drank ad libitum 0.05% sodium iodide aqueous solution for 8 weeks to establish the AIT model， and the control group drank ad libitum distilled water. Eight weeks later， the mice in each dosing group were divided into groups and gavage. The swelling of thyroid tissue was observed with the naked eye， and the weight of spleen was weighed. The content of serum inflammatory factors interleukin-1β （IL-1β）， interleukin-6 （IL-6）， tumor necrosis factor-α （TNF-α） was measured by enzyme-linked immunosorbent assay （ELISA）， and Real-time PCR was used to detect the expression of HMGB1， TLR4， AIM2， NF-κB p65，apoptosis-associated speck-like protein（ASC），cysteinyl aspartate-specific protease-1（Caspase-1）， IL-1β mRNA. Western blot was used to detect the expression of high motility group protein 1 （HMGB1）， TLR4， AIM2， NF-κB p65， phosphorylation（p）-NF-κB p65， ASC， Caspase-1， and IL-1β proteins in thyroid tissue， and immunofluorescence staining was used to observe the protein expression of HMGB1， AIM2， and NF-κB p65 in thyroid tissue of mice. ResultCompared with the control group， the thyroid tissue of mice in the model group was significantly swollen， the spleen quality was significantly increased， and the expression of HMGB1， TLR4， NF-κB p65， AIM2， ASC， Caspase-1， IL-1β in thyroid tissue was significantly increased （P<0.01）. Compared with the model group， the swelling of thyroid tissue in mice in each dose group of Buzhong Yiqitang was improved， the quality of spleen was significantly reduced， and the expression of HMGB1， TLR4， AIM2， NF-κB p65， p-NF-κB p65， ASC， Caspase-1， IL-1β in thyroid tissue was significantly reduced （P<0.05， P<0.01）. ConclusionBuzhong Yiqitang can effectively improve the inflammatory injury of AIT， and regulating the abnormal activation of the TLR4/NF-κB/AIM2 inflammasome signal pathway may be one of its intervention mechanisms.
