Evaluation of Olaparib radiosensitization for breast cancer in nude mice by 18 F-FLT Micro PET / CT imaging
10.19405/j.cnki.issn1000-1492.2023.06.009
- Author:
Siqi Wang
1
;
Weitao Tao
1
;
Alei Xu
1
;
Yangyang Xue
1
;
Hui Wang
1
Author Information
1. Dept of Nuclear Medicine，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
- Publication Type:Journal Article
- Keywords:
breast neoplasms;
poly ( ADP-ribose ) polymerase;
18 F-fluorodeoxythymidine;
radiotherapy;
Micro PET / CT;
Ki-67;
PCNA
- From:
Acta Universitatis Medicinalis Anhui
2023;58(6):930-934
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of 18 F-deoxythymidine nucleoside ( 18 F-FLT) positron emission computed tomography (PET / CT) imaging to evaluate the radiosensitization effect of Olaparib on breast cancer model in nude mice．
Methods :According to the random number table method，twenty-four BALB / C nude mice MCF- 7 breast cancer models were established and divided into four groups with 6 mice in each group，namely the control group，radiotherapy group，Olaparib group and Olaparib + radiotherapy group． 18 F-FLT micro PET / CT imaging was performed on nude mice before and 48 h after treatment，respectively．The changes of maximum standardized uptake value ( SUVmax ) ，total proliferation volume (TPV) and tumor volume before and after tumor treatment in four groups were compared．The tumors were extracted and weighed to observe the changes of tumor weight，and the expression of Ki-67 and PCNA was analyzed by immunohistochemistry staining．The correlation of tumor SUVmax with Ki-67 and PCNA was analyzed.
Results :Before treatment，there were no significant differences in SUVmax ， TPV and tumor volume among the 4 groups (F = 0. 041，0. 061，0. 045，P＞0. 05) ．48 h after treatment，SUVmax in the control and Olaparib groups increased significantly ( t = －12. 111，P ＜0. 001 ; t = －3. 001，P = 0. 03 ) ， SUVmax was reduced in the radiotherapy and Olaparib + radiotherapy groups (t = 5. 829，P＜0. 01 ; t = 4. 448，P < 0. 01) ，while SUVmax ，TPV and tumor volume of tumors in the Olaparib + radiotherapy group were lower than those in the radiotherapy group (t = 3. 388，5. 884，5. 990，P＜0. 01) ．Tumor weight was significantly lower in the Olaparib + radiotherapy group than in the other three groups ( F = 44. 405，P ＜0. 001 ) ． Immunohistochemical staining showed that Ki-67 and PCNA were the least expressed in the Olaparib + radiotherapy group than in the other three groups (F = 16. 289，39. 645，P＜0. 001) ．SUVmax was positively correlated with Ki-67 and PCNA expression (r = 0. 920，0. 918，P＜0. 01) ．
Conclusion :18 F-FLT Micro PET / CT imaging can evaluate the radiosensitizing effect of Olaparib on nude mouse breast cancer model.
- Full text:2024081617125724346_(18)F-FLT_Mi...鼠乳腺癌模型的放疗增敏作用_王思淇.pdf
