The mechanism underlying circHIPK3 promotes the migration and invasion of nasopharyngeal carcinoma cells 5 ⁃8F
10.19405/j.cnki.issn1000-1492.2023.08.017
- Author:
Wenting Shao
1
;
Lei Lv
2
;
Jin Gao
3
Author Information
1. Graduate School , Wannan Medical College , Wuhu 241002
2. Cancer Epigenetics Program , The First Afiliated Hospital of University of Science and Technology of China , Hefei 230031
3. Dept of Radiation Oncology, West Region , The First Afiliated Hospital of University of Science and Technology of China , Hefei 230031
- Publication Type:Journal Article
- Keywords:
ircHIPK3;
5 ⁃8F cells;
nasopharyngeal carcinoma;
migration and invasion;
transcriptome sequencing
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1346-1353
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the role and molecular mechanism of circHIPK3 in the migration and invasion of
nasopharyngeal carcinoma cells.
Methods :Firstly , we searched public databases and analyzed the expression level of circHIPK3 in nasopharyngeal carcinoma tissues and non⁃cancerous tissues in Gene Expression Database (GEO) . Then , the circHIPK3 siRNA was synthesized and transfected into the NPC cell line 5 ⁃8F. After validate the efficiency of circHIPK3 knockdown , the proliferation , migration and invasion capacities of 5 ⁃8F cells were evaluated by CCK⁃8 , wound healing and transwell assay. Meanwhile , the mRNA and protein expression of downstream genes , including CDH1 , CDH2 , MMP2 , MMP9 and IL⁃6/STAT3 were determined by RT⁃qPCR and Western blot. Finally , transcriptome sequencing technology was used to analyze the significantly differentially expressed genes after the interference of circHIPK3 ⁃siRNA in 5 ⁃8F cells , in order to find out the key genes mediating the oncogenesis role of circHIPK3.
Results :circHIPK3 expression significantly increased in nasopharyngeal carcinoma tissues compared with non⁃cancerous tissues ( P < 0. 05 ) . After siRNA⁃specific knockdown of circHIPK3 expression in 5 ⁃8F cells , the proliferation , scratch healing rate , and the number of migrated /invaded cells were significantly reduced (P < 0. 05 , P < 0. 01 , P < 0. 05 respectively) . Meanwhile , the mRNA and protein levels of CDH1 significantly increased (P < 0. 001) , but the CDH2 , MMP2 , MMP9 , IL⁃6/STAT3 mRNA and protein levels were significantly reduced (P < 0. 05) . Further analysis by RNA⁃seq showed that the expression of AL645922. 1 , SCO2 , AL136295. 1 and ISY1 ⁃RAB43 was significantly up⁃regulated( P < 0. 05 ) , and the expression of BIVM⁃ERCC5 , FAM47E⁃ST⁃BD1 , INO80B⁃WBP1 , NAIP , C15orf38⁃AP3S2 , BCL2L2⁃PABPN1 and SMIM11B was significantly down⁃regulated (P < 0. 05) in circHIPK3 ⁃siRNA transfected 5 ⁃8F cells.
Conclusion :circHIPK3 expression is significantly upregulated in nasopharyngeal carcinoma tissues. circHIPK3 promotes 5 ⁃8F cells proliferation , migration and invasion by regulating the expression of E ⁃Cadherin , N ⁃Cadherin , MMP2 , MMP9 and IL⁃6/STAT3. Genes such as AL645922.1 and BIVM⁃ERCC5 may play a key role in promoting the migration and invasion of nasopharyngeal carcinoma 5 ⁃8F cells mediated by circHIPK3.
- Full text:2024081616581914810circHIPK3促进鼻咽...胞5-8F迁移和侵袭的机制_邵文婷.pdf
