SS-31 inhibits O3 -mediated airway hyperresponsiveness and mucus hypersecretion in mice
10.19405/j.cnki.issn1000-1492.2023.06.005
- Author:
Meiqin Xie
1
;
Chenfei Li
2
;
Qi Liu
2
;
Jiali Weng
2
;
Hai Zhang
2
;
Feng Li
2
;
Xiaoyun Fan
1
Author Information
1. Geriatric Respiratory and Critical Care，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
2. Respiratory and Critical Care， Shanghai Chest Hospital，Shanghai Jiao Tong University of Medicine，Shanghai 200030
- Publication Type:Journal Article
- Keywords:
SS-31;
ozone;
oxidative stress;
airway hyperresponsiveness;
mucus hypersecretion
- From:
Acta Universitatis Medicinalis Anhui
2023;58(6):908-913
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate whether Mitochondria-targeted antioxidant peptide SS-31 can inhibit the ozone ( O3 ) -induced mice lung airway hyperresponsiveness and mucus hypersecretion．
Methods :Eight-week C57BL /6 mice were randomized into four groups，including phosphate buffer saline (PBS) + Air group，SS-31 + Air group， PBS + O3 group and SS-31 + O3 group．C57BL /6 mice were injected intraperitoneally with SS-31 ( 10 mg / kg) one hour before ozone exposure ，and then single-exposed to ozone at a concentration of 5. 01 × 10 －6 mol / m3 for 3 hours．After 24 hours，airway hyperresponsiveness(AHR) and bronchoalveolar lavage fluid (BALF) cells numbers were measured．Lung tissue schiff periodic acid shiff (PAS) staining，malondialdehyde (MDA) ，inflammatory factors ( interleukin，IL ) -1 β , IL-6 ，IL-18 and monocyte chemoattractant protein-1 ( MCP-1 ) ) and mucin factor (MUC5B) were detected，and the protein expression levels of NOD-like receptor thermal protein domain associated protein 3 (NLRP3) ，pro-Caspase 1 / Caspase 1 (p20) ，Gasdermin D ( GSDMD) and Cleaved GSDMD were determined by Western blot.
Results:O3 exposure caused both mice lung airway hyperresponsiveness and mucus hypersecretion．However，SS-31 could inhibit the O3 -induced airway hyperresponsiveness and mucus secretion，reduce the levels of oxidative stress and inflammatory factor mRNA expression ，and downregulate the protein expression level of NLRP3 and the activated forms of Caspase 1 and GSDMD．
Conclusion:SS-31 could suppress O3 -induced mice airway hyperresponsiveness and mucus hypersecretion by inhibiting the NLRP3 / Caspase 1 / GSDMD signaling pathway.
- Full text:202408161653539105SS-31抑制O_3介导的小鼠气道高反应和黏液高分泌_谢梅琴.pdf
