Time-dependent expression of ICAM-1 & VCAM-1 on coronaries of the heterotopically transplanted mouse heart.
10.3346/jkms.1999.14.3.245
- Author:
Jeong Ryul LEE
1
;
Jae Hak HUH
;
Jeong Wook SEO
;
Chul Jun SUK
;
Hyang Min JEONG
;
Eul Kyung KIM
Author Information
1. Department of Thoracic and Cardiovascular Surgery, Seoul National University Children's Hospital, Seoul National University College of Medicine, Korea. jrl@plaza.snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
ICAM-1;
VCAM-1;
Heart transplantation;
Coronary atherosclerosis;
Graft rejection
- MeSH:
Animal;
Coronary Vessels/pathology;
Heart Transplantation*/pathology;
Intercellular Adhesion Molecule-1/biosynthesis*;
Mice;
Myocardium/pathology;
Myocardium/metabolism*;
Time Factors;
Transplantation, Heterotopic*/pathology;
Vascular Cell Adhesion Molecule-1/biosynthesis*
- From:Journal of Korean Medical Science
1999;14(3):245-252
- CountryRepublic of Korea
- Language:English
-
Abstract:
To investigate the pathogenesis of accelerated graft atherosclerosis after rdiac transplantation, a genetically well-defined and reproducible animal del is required. We performed heterotopic intraabdominal heart transplantation tween the two inbred strains of mice. Forty hearts from B10.A mice were ansplanted into B10.BR mice. Recipients were sacrificed at 1, 3, 5, 7, 14, 28, d 42 days after implantation. The specimens from both donor and recipient were amined with fluorescent immunohistochemistry and the serial histopathologic anges were evaluated. In the donor hearts, ICAM-1 and VCAM-1 expressions were nimal at day 1 and they gradually increased, reaching their peaks on day 5 or and remained unchanged by day 42. However, there were very little expressions the recipients' hearts. Mean percent areas of intima in the donor coronaries vealed progressive increase by day 42. However, those in the recipients cupied consistently less than 5% of the lumen. In conclusion, we demonstrated at a heterotopic murine heart transplantation model was a useful tool to oduce transplantation coronary artery disease and that adhesion molecules on e cardiac allografts were activated very early and remained elevated at all me-points, nonetheless the arterial lesion was detected after day 28 and its ogression was accelerated thereafter.
