Effect of ACBD3 knockdown on ferroptosis in mammalian cells
10.19405/j.cnki.issn1000-1492.2023.08.016
- Author:
Rong Qiu
1
;
Shanchuan Ma
1
;
Ying Qian
1
;
Xinwang Cao
1
Author Information
1. School of Life Sciences , Anhui Medical University, Hefei 230032
- Publication Type:Journal Article
- Keywords:
ACBD3;
iron homeostasis;
ferroptosis
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1341-1345
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the role of Golgi protein ACBD3 knockdown in ferroptosis.
Methods :ACBD3 knockdown via shRNA , combined with treatment of ferroptosis inducer RSL3 , cell viability , iron levels , reactive oxygen species (ROS) levels , and lipid peroxide levels in different treatment groups were measured to comprehensively analyze the role of ACBD3 in ferroptosis.
Results : Compared to control cells , the levels of ROS increased in ACBD3 knockdown cells. The total iron content was significantly reduced (P < 0. 001) , while the Fe2 + concentration in ACBD3 knockdown cells increased compared to control cells (P < 0. 001) . In the presence of ferroptosis inducer RSL3 , compared to control cells , cell viability significantly decreased ( P < 0. 001) , and lipid peroxide greatly increased in ACBD3 knockdown cells ( P < 0. 001) .
Conclusion :Golgi protein ACBD3 knockdown in mammalian cells changes iron homeostasis and promotes ferroptosis.
- Full text:202408161652449872+.pdf
