Mechanism of TLR7 regulating trigeminal neuralgia in ION⁃CCI model rats through NF⁃ κB inflammatory pathway
10.19405/j.cnki.issn1000-1492.2023.08.015
- Author:
Hongwei Zhang
1
;
Lian Shen
1
;
Beibei Wu
1
;
Yuanyin Wang
1
;
Liecheng Wang
2
Author Information
1. Stomatological College, Anhui Medical University, Hefei 230032
2. School of Basic Medical Sciences ,Anhui Medical University, Hefei 230032
- Publication Type:Journal Article
- Keywords:
Toll⁃like receptor 7;
NF⁃κB;
trigeminal neuralgia;
inflammatory factors
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1334-1340
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To study the role of Toll⁃like receptor 7 (TLR7) in trigeminal nerve pain in SD rats , and to explore the mechanism of TLR7 mediating related inflammatory factors through the activation of NF⁃κB pathway during the process of pain.
Methods :The rat model of trigeminal neuralgia (TN) was established by infraorbital nerve constriction injury(ION⁃CCI) . The expression of TLR7 in trigeminal ganglion (TG) was detected by qRT⁃PCR and Western blot. Hydroxychloroquine (HCQ) , an inhibitor of TLR7 , was given to ION⁃CCI rats by intragastric administration. The expression of TLR7 and its downstream signal pathway NF⁃κB subunit p65 , p ⁃p65 and inflammatory factors (TNF⁃α , IL⁃1β) in TG were detected.
Results :After trigeminal nerve injury induced by ligation of infraorbital nerve , the expression of TLR7 in TG of rats significantly increased. After administration of TLR7 inhibitor, the expression of TLR7 , TNF⁃α and IL⁃1β in TG decreased , the translocation and phosphorylation of p65 nucleus decreased , the activation of NF⁃κB signal pathway was inhibited , and the mechanical pain induced by ION⁃CCI was relieved in male SD rats.
Conclusion :TLR7 in TG regulates neuropathic pain by activating NF⁃κB signal pathway in primary sensory neurons and mediating the expression of inflammatory cytokines TNF⁃α and IL⁃1β .
- Full text:2024081616492476668TLR7通过NF-κB炎症...I模型大鼠三叉神经痛的机制_张宏伟.pdf
