Berberine activates SIRT1 /AMPK signaling pathway to improve mesangial cell proliferation and autophagy function induced by high glucose
10.19405/j.cnki.issn1000-1492.2023.06.003
- Author:
Lin Yang
1
;
Rongrong Wang
1
;
Xiaoyu Guo
1
;
Liqin Tang
2
,
3
;
Wei Wei
1
Author Information
1. Institute of Clinical Pharmacology，Anhui Medical University，Hefei 230032
2. Institute of Clinical Pharmacology，Anhui Medical University，Hefei 230032
3. Dept of Pharmacy，The First Affiliated Hospital of University of Science and Technology of China，Hefei 230001
- Publication Type:Journal Article
- Keywords:
berberine;
diabetic nephropathy;
mesangial cell;
SIRT1 / AMPK;
proliferation;
autophagy
- From:
Acta Universitatis Medicinalis Anhui
2023;58(6):896-901
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of berberine (BBR) on the proliferation and autophagy of mesangial cells in high glucose (HG) environment and the specific molecular mechanism．
Methods:Mesangium cells at exponential growth stage were divided into the following groups : normal group，high glucose group，high glucose + BBR treatment group (30，60 and 90 μmol / L) ，high glucose + BBR (90 μmol / L) + AMPK inhibitor Compound C group ( CC group) ; the number of mesangial cells was calculated by high content cell imager．The expressions of type Ⅳ collagen ( Col-Ⅳ) ，fibronectin (FN) and microtubule-associated protein 1 light chain 3B (LC3B) in mesangial cells were detected by immunofluorescence assay．The protein expression levels of LC3B，Beclin-1， p62，Col-Ⅳ , FN and silencing regulatory factor 1 (SIRT1) / adenylate activated protein kinase (AMPK) signaling pathway were detected by Western blot.
Results:Compared with the normal group ，high content cell imaging showed abnormal proliferation of mesangial cells in the hyperglycemic group．The results of immunofluorescence and Western blot showed that the expression levels of Col-Ⅳ and FN deposited in mesangial extracellular matrix increased in the high glucose group．The results of Western blot showed that the protein expressions of SIRT1，p- AMPK，LC3B and Beclin-1 decreased，while the protein expressions of p-p65 and p62 increased．BBR inhibited the abnormal proliferation of mesangial cells induced by high glucose．BBR could reduce the expression levels of Col-Ⅳ and FN deposited in mesangial extracellular matrix． BBR could increase the expressions of SIRT1 ，p- AMPK，LC3B and Beclin-1 proteins in mesangial cells，while decrease the expressions of p-p65 and p62 proteins. CC group weakened the inhibition of mesangial cell proliferation and autophagy by high dose BBR.
Conclusion:Berberine can effectively inhibit the proliferation of mesangial cells induced by high glucose and increase the level of autophagy，which may be related to SIRT1 / AMPK signaling pathway.
- Full text:2024081616420426395小檗碱激活SIRT1_AM...系膜细胞异常增殖和自噬功能_杨琳.pdf
