Effect of early inhibition of TLR4 on hippocampal immune function to adolescence after neonatal HIBD
10.19405/j.cnki.issn1000-1492.2023.08.012
- Author:
Xiaoli Huang
1
;
Zhicui Ouyang
1
;
Xianghong Wu
2
;
Yan Li
2
;
Yun Huang
2
;
Guoqiong Liu
2
;
Shiwei Lu
2
;
Zhen Tang
1
Author Information
1. Dept of Neonatology, Afiliated Hospital of Guilin Medical University, Guilin 541001
2. Class of2019 Clinical Medicine Pediatrics , Guilin Medical University, Guilin 541001
- Publication Type:Journal Article
- Keywords:
hypoxic⁃ischemic brain damage;
hippocampus;
TLR4;
rat;
neuroimmune
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1317-1322
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the role of early inhibition of Toll⁃like receptor 4 (TLR4) in regulating hippampal neuroimmune responseto adolescent ratsafter neonatal hypoxic⁃ischemic brain damage(HIBD) .
Methods:Postnatal day 7 rats were randomized into controlgroup , hypoxic ischemia (HI) group , and HI + TAK⁃242( the specific inhibitor of TLR4)(TAK⁃242) group. The expression of TLR4 in rat hippocampus was detected by immunohistochemistry at 3 days after HI. Immunofluorescence were used to determine the number of Iba⁃1 + , GFAP + , CD161 + , MPO + and CD3 + cells in the hippocampus at 21 days after HI. Immunohistochemistry was used to detect ICAM⁃1 and C3a expression in the hippocampal CA1 region ; and Western blot was used to detect tumor necrosis factor interleukin IL⁃1β , TNF⁃α and IL⁃10 expression.
Results :Compared with control group , significantly raised TLR4 expression was observed in the left hippocampal CA1 , CA3 and DG regions(P < 0. 01 or P < 0. 05) , while the expression in the TAK⁃242 group lowered compared to the HI group (P < 0. 05) . The number of GFAP + cells in the CA1 area of the hippocampus in the TAK⁃242 group of neonatal rats decreased compared to which in the HI group at 21 days after HI(P < 0. 05) , but the number of CD3 + T lymphocytes in the hippocampal CA1 area of new born rats in the HI group increased compared to which in the Control group (P < 0. 05) , but the difference between TAK⁃242 and the Control group was not statistically significant. The number of Iba⁃1 + cells , MPO + cells , CD161 + cells , the expression of ICAM⁃1 and C3a in hippocampal CA1 region , and the expression of TNF⁃α , IL⁃1β and IL⁃10 in hippocampus of rats were not different among groups at 21 days after HIBD.
Conclusion : Early inhibition of TLR4 may ameliorate adolescent neuroimmune disorders by reducing the increase of hippocampal astrocytesafter neonatal HIBD.
- Full text:2024081616324656658早期抑制TLR4对新生大鼠...青春期脑海马免疫功能的影响_黄晓丽.pdf
