Effects of an ultradian light on periodic expression of glutamate receptors in the SCN and LHB
10.19405/j.cnki.issn1000-1492.2023.08.010
- Author:
Jiayi Li
1
;
Qiaoling Jin
1
;
Liecheng Wang
1
;
Juan Cheng
1
Author Information
1. Dept of Physiology,School of Basic Medical Sciences ,Anhui Medical University,Hefei 230032
- Publication Type:Journal Article
- Keywords:
an ultradian light cycle;
lateral habenula;
suprachiasmatic nucleus;
glutamate receptors
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1306-1312
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the effects of an ultradian light cycle on the periodic expression of glutamate re⁃ ceptors in the suprachiasmatic nucleus and the lateral habenula nucleus.
Methods : An ultradian light cycle T7 (3. 5 hours/3. 5 hours light : dark) was used to establish an ultradian light cycle model group and T24( 12 hours/ 12 hours light : dark) was used to establish control group. The expression of key proteins in the brain regions of the suprachiasmatic nucleus ( SCN) and the lateral habenula ( LHB) of the hypothalamus were analyzed by Western blot. Including glutaminergic receptors , pituitary adenylate cyclase activating polypeptide (PACAP) receptors , and
downstream signaling molecules.
Results :Western blot results showed that the expression of α⁃amino⁃3 ⁃hydroxy⁃5 ⁃methyl⁃4 ⁃isoxazole⁃propionic acid receptor subunit 2 (GluR2) in SCN under Zeitgeber time (ZT ) 1 and ZT 5 increased under an ultradian light cycle compared with normal photoperiod ( P < 0. 05 , P < 0. 01) , the overall expression of GluR2 in T7 group was higher than that in T24 group (P < 0. 01) , the overall expression of GluR2 in
LHB group T7 was lower than that in T24 group (P < 0. 01) . Compared with T24 photoperiod , the overall expression of N ⁃methyl⁃D ⁃aspartic acid receptor subunit 2 (NR2B) in SCN increased under T7 photoperiod (P < 0. 05) , the overall expression of phosphorylation of extracellular signal⁃related kinase ( P⁃ERK) in LHB significantly increased under T7 photoperiod (P < 0. 05) .
Conclusion :An ultradian light cycle would cause an up⁃regulating of GluR2 and NR2B expression , a down⁃regulating of GluR2 expression and up⁃regulating P ⁃ERK expression in LHB.
- Full text:2024081610581837245短时程光照对小鼠SCN和LHB谷氨酸受体表达的影响_李佳逸.pdf
