The study of Sirt1 promotes the osteogenic differentiation of inflammatory PDLSCs
10.19405/j.cnki.issn1000-1492.2023.05.030
- Author:
Xiangwei Kong
1
;
Wei Yin
1
;
Chenchen Wang
1
;
Lin Zhang
1
;
Yicheng Cheng
1
Author Information
1. Dept of Stomatology, the Eastern Theater General Hospital ,Nanjing 210002
- Publication Type:Journal Article
- Keywords:
Sirt1;
inflammatory PDLSCs;
osteogenic differentiation
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):876-880
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the positive effect and mechanism of histone deacetylase Sirtuin 1 (Sirt1) on the osteogenic capacity of inflammatory PDLSCs.
Methods:PDLSCs were isolated and cultured from the healthy individuals and periodontitis patients , the expression of Sirt1 in two types of PDLSCs was observed;osteogenic induction of inflammatory PDLSCs was performed , concurrently Sirt1 agonist resveratrol was used to up⁃regulate the expression of Sirt1 , then the osteogenic differentiation was observed , the expressions of Runx2 , acetylated NF⁃κB , acetylated FoxO1 and FoxO1 were assessed by Western blot.
Results :The protein expression of Sirt1 was suppressed in in flamed PDLSCs( P < 0. 01) ;resveratrol could up⁃regulate the expression of Sirt1 in inflamed PDLSCs;compared with the osteogenic induction group , the osteogenesis + resveratrol group increased the expression of BSP ( t =14. 045 ,P < 0. 01) , Runx2( t = 3. 349 ,P < 0. 01) , OCN( t = 7. 218 ,P < 0. 01) and Osx ( t = 4. 544 ,P < 0. 01) mRNA in inflamed PDLSCs , and enhanced ALP staining(P < 0. 01) ;expression of FoxO1( t = 8. 737 , P < 0. 01) and Runx2( t = 6. 152 , P < 0. 01) increased after osteogenesis of inflamed PDLSCs ; in the osteogenesis + resveratrol
group , the expression of Sirt1 was up⁃regulated , and the expression of FoxO1( t = 5. 912 ,P < 0. 01) and Runx2( t =6. 277 ,P < 0. 01) further increased compared with the osteogenic induction group ,while the expression of acetylated NF⁃κB(F = 184. 033 ,P < 0. 01) and acetylated FoxO1(F = 301. 454 , P < 0. 01) was significantly lower than that of control group and osteogenic induction group.
Conclusion:The deacetylase Sirt1 could promote the osteogenic differentiation of inflammatory PDLSCs through deacetylating NF⁃κB and FoxO1 .
- Full text:2024081419041707780Sirt1促进炎症牙周膜干细胞成骨分化的机制_孔祥伟.pdf
