Mechanism of celecoxib inhibiting the expression of retinal VEGF in diabetic retinopathy rats via JAML
10.19405/j.cnki.issn1000-1492.2023.08.008
- Author:
Mei Duan
1
;
Fan Cao
1
;
Yanchao Gui
1
;
Keyang Chen
2
;
Liming Tao
1
;
Zhengxuan Jiang
1
Author Information
1. Dept of Ophthalmology, The Second Afiliated Hospital of Anhui Medical University, Hefei 230601
2. Dept of Inspection and Quarantine Health , Anhui Medical University, Hefei 230032
- Publication Type:Journal Article
- Keywords:
celecoxib;
JAML;
diabetes retinopathy;
phosphatidylinositol
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1293-1299
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect and mechanism of celecoxib on retinal vascular endothelial growth factor(VEGF) in rats with diabetic retinopathy.
Methods : Forty - five SD rats were divided into normal control group (NC) , diabetic retinopathy group (DR) , celecoxib intervention diabetic retinopathy group ( DR + C) . The diabetic model was established by intraperitoneal injection of 1% STZ. After one month , celecoxib (50 mg/kg) was given intragastric administration (1/day) in the DR + C group. Two months later, serum total cholesterol (TC) and insulin were detected. The histopathological changes of the retina were observed. The expression of junctional adhesion molecule⁃like protein (JAML) , VEGF and their signal pathway proteins and the distributions of interleukin⁃10 (IL⁃10) , vascular cell adhesion molecules⁃1(VCAM⁃1) were detected by Western blot. HUVEC cells were divided into normal glucose group (NG) , high glucose group (HG) and high glucose plus celecoxib group (HG + C) to detect the expression of the above proteins.
Results :Compared with DR , retina in DR + C group was thinner. The retina in the DR + C group was thicker than that in the NC group. The levels of retinal JAML ,phosphatidylinositol kinase3(PI3K), phosphorylphosphatidylinositol kinase3(P⁃PI3K) , hypoxia⁃inducible factor1 ⁃α (HIF1 ⁃α ) , and VEGF in DR + C group were lower than those in DR group ,while higher than those in NC group. The expression of retinal IL⁃10 and VCAM⁃1 decreased . The content of TC in DR + C and DR group was higher than those in NC group (P < 0. 01) , while the content of insulin in DR + C and DR group was lower than thlse in NC group (P < 0. 001) . Compared with HG group , the expressions of JAML , PI3K , P ⁃PI3K , HIF1 ⁃α , VEGF in HG + C group decreased , but was higher than those in NG group. There was no significant difference in PI3K among the three groups.
Conclusion :Celecoxib can decrease the expression of VEGF , IL⁃10 , VCAM⁃1 in retina of DR rats , which may be related to the PI3K/HIF1 ⁃α signaling pathway mediated by JAML.
- Full text:2024081417303524818塞来昔布下调JAML抑制糖...大鼠VEGF的表达及其机制_段梅.pdf
