LC⁃MS/MS simultaneous determination of 22 bile acids in serum , liver , amniotic fluid and placenta of pregnant mice
10.19405/j.cnki.issn1000-1492.2023.08.007
- Author:
Fan Zhao
1
,
2
,
3
;
Lun Zhang
3
,
4
;
Lu Ye
1
,
2
,
3
;
Jiayi Zhang
1
,
2
,
3
;
Yun Yu
3
,
4
;
Qianqian Huang
3
,
4
;
Jianqing Wang
1
,
2
,
3
Author Information
1. School of Pharmacy, Anhui Medical University, Hefei 230032
2. Dept of Pharmacy, The First Afiliated Hospital of Anhui Medical University, Hefei 230012
3. Anhui Public Health Clinical Center, Hefei 230012
4. Dept of Pharmacy, The First Afiliated Hospital of Anhui Medical University, Hefei 230012
- Publication Type:Journal Article
- Keywords:
bile acids;
high performance liquid chromatography⁃mass spectrometry system;
pregnant mice;
methodology
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1287-1292,1299
- CountryChina
- Language:Chinese
-
Abstract:
Objective :High performance liquid chromatography⁃mass spectrometry (LC⁃MS/MS) system was used to accurately determine 22 bile acids in serum , liver, amniotic fluid and placenta of pregnant mice , and a LC⁃MS/ MS method was established for efficient detection and analysis of bile acids in serum , liver, amniotic fluid and placenta of mice.
Methods :Pregnant mice serum , liver, amniotic fluid and placenta samples were processed , with 0. 1% glacial acetic acid in 4 mmol/L ammonium acetate aqueous solution as mobile phase A and pure methanol as
mobile phase B , the flow rate was 0. 4 ml/min , a gradient elution program was used to elute with Phenomenex Gemini 3 μm NX⁃C18 110A ( 100 mm × 2. 0 mm) chromatographic column elution , and mass spectrometry detection system used an electrospray ion source for negative ion multiple reaction monitoring.
Results :The linear relationship of 22 bile acids in the quantitative range was good. The RSD of inter⁃day and intra⁃day precision at low , medium and high concentrations was 0. 5% - 7. 4% , the matrix effect was 88% - 110% , and the extraction recovery was 84% - 108% .
Conclusion : In this experiment , LC⁃MS/MS was established to detect 22 bile acids in serum , liver, amniotic fluid and placenta of pregnant mice. The method not only has high sensitivity and selectivity , but also can stably detect a large number of samples.
- Full text:2024081417053092119LC-MS_MS同时测定2...、肝脏、羊水和胎盘中的应用_赵凡.pdf
