Effect of hypoxia/cold exposure on white fat browning in a rat model of obesity
10.19405/j.cnki.issn1000-1492.2023.08.005
- Author:
Kun Gao
1
,
2
;
Lixin Yang
1
,
2
;
Ye Wang
1
,
2
;
Haiqi Xu
1
,
2
Author Information
1. Dept ofEndocrinology , Qinghai Provincial People &prime
2. s Hospital , Xining 810007
- Publication Type:Journal Article
- Keywords:
hypoxia;
cold exposure;
peroxisome proliferator⁃activated receptor γ2;
PR structural domain binding factor;
uncoupling protein 1
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1274-1279
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of hypoxia/cold exposure on white fat browning in an obese rat
model .
Methods :Obese rat model was constructed by high⁃fat feeding and randomly divided into control group ,
of body weight and body fat were observed , and the morphological changes of rat adipose tissue cells were observed using HE staining and compared with the area of adipocytes . The expression of peroxisome proliferator⁃activated receptor γ2 (PPAR⁃γ2) , PR structural domain binding factor 16 ( PRDM16) and uncoupling protein 1 ( UCP⁃1) gene and the expression of UCP⁃1 protein in the adipose tissue of rats were examined by immunofluorescence , realtime fluorescence quantitative PCR and Western blot .
Results :Compared with the control group , the body weight and body fat of rats in the hypoxic , hypothermic and cryogenic hypoxic groups were reduced , and the body weight and body fat of rats in the hypothermic hypoxic group were lower than those in the hypoxic and cryogenic groups (P< 0. 05) . Compared with the control group , the adipocyte area of rats in the hypoxia , hypothermia and low⁃temperature hypoxia groups was reduced , and the adipocyte area of rats in the low⁃temperature hypoxia group was lower than that in the hypoxia and low⁃temperature groups (P < 0. 05) . Compared with the control group , the expression of PPAR⁃γ2 , PRDM16 and UCP⁃1 genes all increased in the scapular brown adipose tissue ( F = 378. 495 , 102. 061 , 322. 443 , P < 0. 05) and decreased in the perirenal white adipose tissue (F = 4. 555 , P < 0. 05) in the hypoxic , hypothermic and low⁃temperature hypoxic groups of rats , PRDM16 and UCP⁃1 gene expression all increased (F = 24. 387 , 163 . 660 , P < 0. 05) . Compared with the control group , the expression of UCP⁃1 protein in scapular brown adipose tissue and perirenal white adipose tissue of rats in the hypoxic , hypothermic and low⁃temperature hypoxic groups increased (P < 0. 05) ; UCP⁃1 protein expression was lower in scapular brown adipose tissue and perirenal white adipose tissue of rats in the hypoxic and hypothermic groups compared to the hypothermic hypoxic group (P < 0. 05) .
Conclusion : Hypoxia/cold exposure can induce white adipose browning and affect the
body weight of rats by modulating the intra⁃adipose PPAR⁃γ2 and PRDM16 pathways which lead to high UCP⁃1 expression .
- Full text:2024081410412928486低氧_冷暴露对肥胖大鼠模型白色脂肪棕色化的影响_郜琨.pdf
