Genetic characterization and potential transmission risk of novel IncpGRT1 plasmids from Pseudomonas species
10.19405/j.cnki.issn1000-1492.2023.08.003
- Author:
Xinyue Li
1
,
2
;
Peng Wang
3
;
Fangzhou Chen
3
;
Xiaofei Mu
3
;
Xiuhui Lu
3
;
Jiaqi He
3
;
Yali Zheng
3
Author Information
1. School of Basic Medical Sciences , Anhui Medical University , Hefei 230032
2. State Key Laboratory ofPathogen and Biosecurity , Beijing Institute ofMicrobiology and Epidemiology , Academy ofMilitary Medical Sciences , Beijing 100071
3. State Key Laboratory of Pathogen and Biosecurity , Beijing Institute ofMicrobiology and Epidemiology , Academy ofMilitary Medical Sciences , Beijing 100071
- Publication Type:Journal Article
- Keywords:
Pseudomonas;
mobile element;
plasmid;
comparative genomics
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1261-1266
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To analyze the genome structure and genetic characteristics of IncpGRT1 plasmids from Pseud⁃
omonas , and elucidate its potential transmission risk .
Methods :The genomic DNA of the clinical isolate 15420352 was extracted after purification and preservation of the strain , and then the whole genome was sequenced , and then the type of the plasmid was identified . Sequence annotation and comparison of the backbone region and the accessory modules were performed on all five same type sequenced plasmids , including one plasmid p420352 - strA in this
study and four from GenBank . The plasmids were annotated by RAST , Plasmidfinder , Blast , ResFinder , and ISfinder. The ORFs of the plasmid were annotated and drug resistance genes were found .
Results :All five plasmids were classified as new IncpGRT1 type plasmids . The IncpGRT1 backbone genes or gene loci were in all five plasmids , and they contained an auxiliary replicon besides the primary IncpGRT1 replicon . Five IncpGRT1 plasmids carried at least three different accessory modules , including the srp region , the msr region , and a Tn5053 family transposon . Three
resistance genes strA , strB , and mer were obtained in these plasmids , which were involved in resistance to two categories of antibiotics and heavy metals . We also found that these plasmids carried at least one virulence gene msr and five key transporters srp , emrE , mod , phn , and lpt , which could improve the environmental adaptability of the strains .
Conclusion :The IncpGRT1 plasmids have become the important vector for the accumulation and spread of some drug resistance genes and virulence genes in Pseudomonas , and have improved the environmental adaptability of the strain.
- Full text:2024081410271691824假单胞菌属中新型Inc_(...的遗传特性及其潜在传播风险_李昕悦.pdf
