HCAR2 mediates hypoxia⁃promoted erythroid differentiation of K562 cells
10.19405/j.cnki.issn1000-1492.2023.08.001
- Author:
Ying Chen
1
,
2
;
Jian Li
3
;
Yan Cao
4
;
Yunling He
4
;
Liying Wu
4
;
Gangqiao Zhou
1
,
2
Author Information
1. School of Life Sciences , Anhui Medical University, Hefei 230032
2. National Center for Protein Sciences , State Key Lab of Proteomics , Institute of Radiation Medicine , Academy ofMilitary Medical Sciences , Academy of Military Sciences , Beijing 100850
3. Institute ofMicrobiology and Epidemiology , Academy ofMilitary Medical Sciences , Academy ofMilitary Sciences , Beijing 100850
4. National Center for Protein Sciences , State Key Lab of Proteomics , Institute of Radiation Medicine , Academy ofMilitary Medical Sciences , Academy of Military Sciences , Beijing 100850
- Publication Type:Journal Article
- Keywords:
erythroid differentiation;
hypoxia;
HCAR2;
K562 cell
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1249-1255
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the effect of hydroxy⁃carboxylic acid receptor 2 (HCAR2) on erythroid differen⁃
tiation of human chronic myeloid leukemia K562 cells under hypoxia .
Methods :The cells were cultured in medium containing hemin to compare the ability of K562 cells to differentiate into erythroid cells under normoxia/hypoxia conditions . Then the expression of HCAR2 was interfered with genetic methods to knockdown or overexpress it in K562 cells , respectively . Benzidine staining , RT⁃qPCR , Western blot and flow cytometry were used to detect the role of HCAR2 in the erythroid differentiation of K562 promoted by hypoxia .
Results : On day 1 , 2 , 3 after differentiation , the expression level of HCAR2 and the erythroid differentiation， the expression level of HCAR2 and the erythroid differentiation ability of K562 cells increased gradually with time , and hypoxia enhanced the above phenotypes compared with normoxia . In HCAR2⁃silenced K562 cells , erythroid cells was reduced and the expression of erythroid differentiation markers CD235a and γ⁃globin decreased . Simultaneously , the proportion of double⁃positive cells expressing erythroid cell surface molecules CD71 and CD235a was lower than that of the control group . On the contrary , the overexpression of HCAR2 in K562 cells increased erythroid cells generation as well as elevated the expression levels of CD235a and γ⁃globin . In addition , the proportion of CD71 /CD235a double⁃positive cells also increased in HCAR2⁃overexpressed K562 cells .
Conclusion :HCAR2 mediates hypoxia in promoting the erythroid differentiation of K562 cells .
- Full text:2024081410170511255HCAR2介导低氧促K562细胞向红系分化作用_陈迎.pdf
