A rapid tigecycline and polymyxin B susceptibility test for Klebsiella pneumoniae using direct⁃on⁃target micro⁃droplet growth assay
10.19405/j.cnki.issn1000-1492.2023.05.026
- Author:
Zhaoxin Xia
1
;
Wensu Yang
1
;
Yi Zhu
1
;
Xinyi Hu
1
;
Chunhui Lin
1
;
Tong Jiang
1
;
Jilu Shen
1
Author Information
1. The First Afiliated Hospital of Anhui Medical University, The Anhui Public Health Clinical Center,Laboratory Dept ,Hefei 230022
- Publication Type:Journal Article
- Keywords:
DOT⁃MGA;
antibiotic susceptibility test rapid assay;
tigecycline;
polymyxin
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):859-862
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the direct⁃on⁃target micro⁃droplet growth assay (DOT⁃MGA) for rapid detection of the susceptibility of tigecycline and polymyxin.
Methods:A total of 67 strains of Klebsiella pneumoniae were collected for DOT⁃MGA. 6 μl droplets with or without tigecycline or polymyxin (The final drug concentration is 2 μg/ml) were added in triplicate into the wells of the MALDI plate with four incubation time points (3 h ,4 h ,6 h , and 8 h) . The results were classified as susceptible (score < 1. 7) and non⁃susceptible (score≥1 . 7) according to the Bruker Biotype software
Results:After incubation for 4 h , the growth efficiency, specificity and positive predictive value of tigecycline and polymyxin were both 100. 00% . The classification consistency rate was 98. 15% and 96. 15% , the sensitivity was 96. 30% and 92. 31% , and the negative predictive value was 96. 45% and 92. 86% ,
respectively.
Conclusion :DOT⁃MGA can provide rapid and reliable drug susceptibility diagnosis of tigecycline and polymyxin ,which is of great significance for the clinical anti⁃infective treatment.
- Full text:2024081322045751275直接靶板微滴法快速检测肺炎...替加环素和多黏菌素的敏感性_夏兆新.pdf
